GCC185 Antibody - Azide and BSA Free

Western Blot: GCC185 Antibody [NBP3-03436] - Analysis of extracts of various cell lines, using GCC185 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP3-03436] - Paraffin-embedded mouse spleen using GCC185 antibody at dilution of 1:150 (40x lens).

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP3-03436] - Paraffin-embedded human colon using GCC185 antibody at dilution of 1:150 (40x lens).

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP3-03436] - Paraffin-embedded human stomach using GCC185 antibody at dilution of 1:150 (40x lens).

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP3-03436] - Paraffin-embedded human appendix using GCC185 antibody at dilution of 1:150 (40x lens).

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GCC185 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCC185 (NP_852118.1). MEDLVQDGVASPATPGTGKSKLETLPKEDLIKFAKKQMMLIQKAKSRCTELEKEIEELRSKPVTEGTGDIIKALTERLDALLLEKAETEQQCLSLKKENIKMKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCC2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for GCC185 Antibody - Azide and BSA Free

• 185 kDa Golgi coiled-coil protein
• CLL-associated antigen KW-11
• CTCL tumor antigen se1-1
• GCC protein, 185-kD
• GCC185GRIP and coiled-coil domain-containing 2
• Golgi coiled-coil protein GCC185
• GRIP and coiled-coil domain containing 2
• GRIP and coiled-coil domain-containing protein 2
• KIAA0336REN53
• Ran-binding protein 2-like 4
• RANBP2L4
• Renal carcinoma antigen NY-REN-53

Background

GCC185 is encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: GCC2