Golgi Glycoprotein 1/GLG1 Antibody (858238) [Unconjugated]

Western blot shows lysates of MCF-7 human breast cancer cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Golgi Glycoprotein 1/GLG1 Monoclonal Antibody (Catalog # MAB78791) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Golgi Glycoprotein 1/GLG1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Golgi Glycoprotein 1/GLG1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse Golgi Glycoprotein 1/GLG1 Monoclonal Antibody (Catalog # MAB78791) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 858238
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Golgi Glycoprotein 1/GLG1 Antibody (858238) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Golgi Glycoprotein 1/GLG1
  Lys1048-Asn1145
  Accession # Q92896
• Specificity: Detects human Golgi Glycoprotein 1/GLG1 in direct ELISA. Detects human and mouse GLG1 in Western Blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLG1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Golgi Glycoprotein 1/GLG1 Antibody (858238) [Unconjugated]

• CFR1
• CFR-1
• cysteine-rich fibroblast growth factor receptor
• DKFZp686L08213
• E-selectin ligand 1
• ESL1
• ESL-1
• FLJ23319
• FLJ23967
• FLJ32797
• GLG1
• Golgi apparatus protein 1
• Golgi Glycoprotein 1
• golgi sialoglycoprotein MG-160
• MG160
• MG-160

Background

GLG1 (Golgi complex-Localized Glycoprotein 1), also known as CFR1, E-Selectin Ligand-1/ESL-1, MG-160 and Cys-rich FGF Receptor, is a 150-160 kDa (reducing; 130 kDa nonreducing) glycoprotein. It is expressed in both Golgi and/or the cell membrane of multiple cell types, including neutrophils (from rodents; not humans), liver stellate cells, neurons, cardiac myocytes, monocytes and bronchial epithelial cells. In the blood, GLG1/ESL-1 collaborates with PSGL-1 to mediate leukocyte binding to endothelial cell surfaces. PSGL-1 initiates leukocyte tethering while GLG1 promotes slow rolling. GLG1 also serves as an intra-Golgi receptor for multiple FGFs, including FGF-1, -2, -4, -18 and possibly -3, and as a component of an unusual latent TGF-beta complex. Mature human GLG1 is an 1150 amino acid (aa) type I transmembrane protein. It contains a 1116 aa extracellular/luminal region (aa 30-1145) plus a short 13 aa cytoplasmic segment. The extracellular region possesses a 16 aa poly-Gln segment followed by 16 Cys-rich repeats (aa 116-1101). There are three potential isoform variants, one of which possess a 24 aa extension at the C‑terminus, a second that couples the aforementioned C‑terminal extension to a deletion of aa 147-157, and a third that contains a 14 aa substitution for aa 685-1179. It is suggested that the longer C‑terminus retains GLG1 in the Golgi, while shorter cytoplasmic segments allow for presentation at the cell membrane. Over aa 1048‑1145, human and mouse are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Golgi Glycoprotein 1/GLG1 Antibody (MAB78791)(1)

Publication using MAB78791

• Kai-Min Lin, Katie Nightingale, Lior Soday, Robin Antrobus, Michael P. Weekes Rapid Degradation Pathways of Host Proteins During HCMV Infection Revealed by Quantitative Proteomics Frontiers in Cellular and Infection Microbiology 2021-01-27 [PMID: 33585265]

Bioinformatics

• Gene Symbol: GLG1
• Uniprot:
  • Q92896()