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GODZ Antibody [HRP]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-06315AF350
Alexa Fluor 405
NBP3-06315AF405
Alexa Fluor 488
NBP3-06315AF488
Alexa Fluor 532
NBP3-06315AF532
Alexa Fluor 594
NBP3-06315AF594
Alexa Fluor 647
NBP3-06315AF647
Alexa Fluor 700
NBP3-06315AF700
Alexa Fluor 750
NBP3-06315AF750
Biotin
NBP3-06315B
DyLight 350
NBP3-06315UV
DyLight 405
NBP3-06315V
DyLight 488
NBP3-06315G
DyLight 550
NBP3-06315R
DyLight 594
NBP3-06315DL594
DyLight 650
NBP3-06315C
DyLight 680
NBP3-06315FR
DyLight 755
NBP3-06315IR
Unconjugated
NBP3-06315-100ul
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GODZ Antibody [HRP] Summary

Immunogen
Produced in rabbits immunized with E. coli-derived Human GODZ fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZDHHC3
Purity
Antigen and protein A Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen and protein A Affinity-purified

Alternate Names for GODZ Antibody [HRP]

  • DHHC domain containing 3
  • DKFZp313D2314
  • EC 2.3.1
  • EC 2.3.1.-
  • FLJ20209
  • FLJ45940
  • Protein DHHC1
  • ZDHC3
  • zinc finger, DHHC-type containing 3

Background

Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: ZDHHC17 Antibody [NBP1-81748] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Western Blot: ZDHHC17 Antibody [NBP1-81748] - Analysis in control (vector only transfected HEK293T lysate) and ZDHHC17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ZDHHC17 Antibody [NBP1-81748] - Staining of human cerebral cortex shows moderate cytoplasmic and membranous positivity in neurons.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
ZDHHC13 Antibody
NBP1-51920
Western Blot: ZDHHC13 Antibody [NBP1-51920] - Analysis of ZDHHC13 in human cerebellum lysate (35ug protein in RIPA buffer) using NBP1-51920 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ZDHHC13 Antibody [NBP1-51920] - staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
TRAILR1/TNFRSF10A
Antibody [Unconjugate ...
AF347
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R1/TNFRSF10A Fc Chimera (Catalog # 347‑DR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375‑TL) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (12 ng/mL) activity elicited by Recombinant Human TRAIL R1/TNFRSF10A Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL R1/TNFRSF10A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF347). The ND50 is typically 0.02‑0.055 µg/mL.
Western blot shows lysates of TF‑1 human erythroleukemic cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL R1/TNFRSF10A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF347) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRAIL R1/TNFRSF10A at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
TRAIL R1/TNFRSF10A was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human TRAIL R1/TNFRSF10A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF347) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(34 Publications)
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Applications: IHC, Neut, WB
ARFGEF2 Antibody
NBP1-88607
Western Blot: ARFGEF2 Antibody [NBP1-88607] - Analysis in human cell line CACO-2 and human cell line MCF-7.
Immunohistochemistry-Paraffin: ARFGEF2 Antibody [NBP1-88607] - Staining of human small intestine shows distinct cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
PrPC Antibody
NB100-56605
Western Blot: PrPC Antibody [NB100-56605] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 162-170 and (B) fusion partner without these amino acids, using this antibody at 5 ug/ml.
Species: Av, Bv, Sh
Applications: WB
Radixin Antibody
(1D9)
H00005962-M06
Western Blot: Radixin Antibody (1D9) [H00005962-M06] - Analysis of RDX expression in HeLa (Cat # L013V1).
Immunohistochemistry: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. Merged image of radixin and phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fncel.2013.00161/abstract) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Analysis of monoclonal antibody to RDX on HeLa cell. Antibody concentration 30 ug/ml
(4 Publications)
Species: Ch, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, WB
NCOA6 Antibody
NB200-335
Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Applications: IHC, IHC-P, IP, WB
Ubiquilin 1
Antibody
NB120-3341
Western Blot: Ubiquilin 1 Antibody [NB120-3341] - Analysis of ubiquilin on CHO cell extract.
(1 Review)
Species: ChHa, Ha, Hu, Mu
Applications: WB
GABA-A R gamma 2
Antibody
NB300-190
Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190] - Immunostaining of mouse retina showing specific labeling of the GABAa gamma-2 subunit in green, calbindin in red and DNA in blue. Photo courtesy of Dr. Arlene Hirano, UCLA.
Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.
(15 Publications)
Species: Hu, Mu, Rt
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Phospholipase C
like 1 Antibody
NBP1-80896
Immunohistochemistry-Paraffin: Phospholipase C like 1 Antibody [NBP1-80896] - Staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.
Immunohistochemistry-Paraffin: Phospholipase C like 1 Antibody [NBP1-80896] - Staining of human heart muscle shows moderate cytoplasmic positivity.
Immunohistochemistry-Paraffin: Phospholipase C like 1 Antibody [NBP1-80896] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Species: Hu
Applications: IHC, IHC-P
TRAILR2/TNFRSF10B
Antibody
NB100-56618
Immunohistochemistry-Paraffin: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis of a formalin fixed paraffin-embedded (FFPE) human brain cerebellum using 1:200 conc. of TRAIL R2/TNFRSF10B antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in the Purkinje cell layer.
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody [NB100-56618] - Pharmacodynamic assays for ONC201. IHC analyses of CHOP, TRAILR2/TNFRSF10B, and double-stranded DNA breaks (TUNEL) in baseline and ONC201-treated biopsies for an endometrial cancer patient. The biopsy was done 9.8 months after starting ONC201 treatment (7 days after the most recent ONC201 dose). Image collected and cropped by CiteAb from the following publication (https://jitc.biomedcentral.com/articles/10.1186/s40425-019-0599-8) licensed under a CC-BY licence.
Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis using the Azide Free version of NB100-56618. Detection of 20 ug of whole cell lysates from HL60 cells with anti-TRAIL-R2 at 5 ug/ml.
(1 Review)
(41 Publications)
Species: Hu, Mu
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, WB
Huntingtin Antibody
NBP3-13106
Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.
Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Huntingtin antibody (NBP3-13106) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Huntingtin protein stained by Huntingtin antibody (NBP3-13106) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Paralemmin Antibody
NBP1-87770
Immunohistochemistry-Paraffin: Paralemmin Antibody [NBP1-87770] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Western Blot: Paralemmin Antibody [NBP1-87770] - Analysis in control (vector only transfected HEK293T lysate) and PALM over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: Paralemmin Antibody [NBP1-87770] - Staining of human cell line HEK 293 shows localization to nucleus & plasma membrane. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
GABARAP Antibody -
BSA Free
NBP1-71771
Immunocytochemistry/Immunofluorescence: GABARAP Antibody [NBP1-71771] - GABARAP antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: GABARAP Antibody [NBP1-71771] - Analysis of GABARAP in mouse liver using DAB with hematoxylin counterstain.
Immunocytochemistry/Immunofluorescence: GABARAP Antibody [NBP1-71771] - analysis of GABARAP in Raw 264.7 cells using anti-GABARAP antibody (red). Nuclei were counterstained with DAPI (blue).
(3 Publications)
Species: Dr, Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
ALS2CR3 Antibody
(S390-43)
NBP2-59333
Immunocytochemistry/Immunofluorescence: ALS2CR3 Antibody (S390-43) [NBP2-59333] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trak2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X.
Western Blot: ALS2CR3 Antibody (S390-43) [NBP2-59333] - Analysis of Rat Brain-Membrane showing detection of ~ 100 kDa TRAK2 protein using Mouse Anti-TRAK2 Monoclonal Antibody, Clone S390-43. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug protein. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-TRAK2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 100 kDa.
Species: Hu, Rt
Applications: ICC/IF, IHC, WB
APE Antibody
(13B8E5C2) - BSA Free
NB100-116
Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-APE (13B8E5C2) at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Chromatin Immunoprecipitation (ChIP): APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Association of p53 and APE1 on p53-binding sites in p21 promoter. Re-ChIP analysis (first IP with alpha-APE1 and the second IP with alpha-p53 antibody) showing simultaneous recruitment of APE1 and p53 in control vs. EPE treated cells; *: p value <0.05 (n=2) calculated based on APE1/p53 enriched DNA from control vs. etoposide treated cells. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY licence.
(3 Reviews)
(102 Publications)
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, PLA, Simple Western, WB
HAP1 Antibody (1B6)
NB110-74569
Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.
Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Detection of HAP1 in mouse brain lysate.
Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.
(1 Review)
(8 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
GODZ Antibody [HRP]
NBP3-06315H
GODZ Antibody [HRP]
Species: Hu
Applications: WB

Publications for GODZ Antibody (NBP3-06315H) (0)

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Bioinformatics

Gene Symbol ZDHHC3