GNB1 Antibody - BSA Free

Western Blot: GNB1 Antibody [NBP2-16699] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Mouse testis. GNB1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Human lung papillory adenocarcinoma, using GNB1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation: GNB1 Antibody [NBP2-16699] - Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 ug of GNB1 antibody. Western blot analysis was performed using GNB1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry Whole-Mount:GNB1 Antibody [NBP2-16699] -Analysis (whole mount) of zebrafish embryo, using GNB1 antibody at 1:200 dilution.

Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (NBP2-16699) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GNB1 antibody detects GNB1 protein at cell membrane in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. GNB1 antibody (NBP2-16699) diluted at 1:1000. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (NBP2-16699) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GNB1 protein stained by GNB1 antibody (NBP2-16699) diluted at 1:500.Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000.Blue: Hoechst 33342 staining.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (NBP2-16699) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GNB1 antibody (NBP2-16699) at 1:300 dilution.

• Reactivity: Hu, Mu, Rt, Ze, Bv, Ca, Ch, Gp, RM, Xp
• Applications: WB, Func, ICC/IF, IHC, IP, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GNB1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GNB1 Antibody - BSA Free (NBP2-16699) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary.
• Predicted Species: Chicken (96%), Canine (100%), Xenopus (96%), Rhesus Macaque (100%), Bovine (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Whole-Mount Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Proximity Ligation Assay Assay dependent
  • Western Blot 1:1000-1:10000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GNB1 Antibody - BSA Free

• beta subunit, signal-transducing proteins GS/GI
• G protein, beta-1 subunit
• guanine nucleotide binding protein (G protein), beta polypeptide 1
• guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1
• guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• Transducin beta chain 1

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GNB1