GNB1 Antibody

Images

 
Western Blot: GNB1 Antibody [NBP2-16699] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: GNB1 Antibody [NBP2-16699] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Immunohistochemical analysis of paraffin-embedded Lung papillory AdCA, using antibody at 1:500 dilution.
Western Blot: GNB1 Antibody [NBP2-16699] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GNB1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary.
Predicted Species
Rat (100%), Chicken (96%), Canine (100%), Xenopus (96%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (96%), Xenopus tropicalis (96%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GNB1 Antibody

  • beta subunit, signal-transducing proteins GS/GI
  • G protein, beta-1 subunit
  • guanine nucleotide binding protein (G protein), beta polypeptide 1
  • guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1
  • guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
  • Transducin beta chain 1

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GNB1