Glypican 6 Antibody (348701) [Unconjugated]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glypican 6 Monoclonal Antibody (Catalog # MAB2845, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 348701
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Glypican 6 Antibody (348701) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glypican 6
  Asp24-Val527
  Accession # Q9Y625
• Specificity: Detects human Glypican 6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Glypican 2, 3, or 5 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPC6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 6 Antibody (348701) [Unconjugated]

• Glypican 6
• glypican proteoglycan 6
• glypican-6
• GPC6
• MGC126288
• OMIMD1

Background

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1-4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C‑terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is down‑regulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9).

1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241. 
2. Filmus, J. (2001) Glycobiology 11:19R. 
3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117. 
4. Filmus, J. (2003) Glycoconj. J. 19:319. 
5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
6. Paine-Saunders, S. et al. (1999) Genomics 57:455.
7. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
8. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
9. Bassett, J.H.D. et al. (2006) Endocrinology 147:295.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 6 Antibody (MAB2845)(1)

Publication using MAB2845

• Julien Cherfils‐Vicini, Charlene Iltis, Ludovic Cervera, Sabrina Pisano, Olivier Croce, Nori Sadouni et al. Cancer cells induce immune escape via glycocalyx changes controlled by the telomeric protein TRF 2 The EMBO Journal 2019-06-03 [PMID: 31000523]

Bioinformatics

• Gene Symbol: GPC6
• Uniprot:
  • Q9Y625()