Glypican 4 Antibody (51E3)

Images

 
Western Blot: Glypican 4 Antibody (51E3) [NBP2-49892] - Glypican 4 Antibody (AT51E3) [NBP2-49892] - The Recombinant Human GPC4 (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and ...read more
Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody (51E3) [NBP2-49892] - Glypican 4 Antibody (AT51E3) [NBP2-49892] - analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and ...read more
Flow Cytometry: Glypican 4 Antibody (AT51E3) [NBP2-49892] - Analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
51E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Glypican 4 Antibody (51E3) Summary

Immunogen
Recombinant human GPC4(401-529aa) purified from E. coli
Specificity
Anti-human GPC4 mAb, clone AT51E3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GPC4 protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GPC4
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA
  • Flow Cytometry 1:200
  • Immunocytochemistry/Immunofluorescence 1:200

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Glypican 4 Antibody (51E3)

  • Glypican 4
  • glypican proteoglycan 4
  • glypican-4
  • GPC4
  • K-glypican
  • K-glypicandJ900E8.1 (glypican 4)

Background

The glypican (Gpc) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the stage of intiation of neural tube closure, suggesting that glypican-4 is unlikely to play an essential role in convergent extension in the mouse

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GPC4
Entrez