Glypican 4 Antibody (51E3) - BSA Free

Western Blot: Glypican 4 Antibody (51E3) [NBP2-49892] - Glypican 4 Antibody (AT51E3) [NBP2-49892] - The Recombinant Human GPC4 (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human GPC4 Lane 2. : Ramos cell lysate

Immunocytochemistry/Immunofluorescence: Glypican 4 Antibody (51E3) [NBP2-49892] - Glypican 4 Antibody (AT51E3) [NBP2-49892] - analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: Glypican 4 Antibody (51E3) [NBP2-49892] - Analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 51E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Glypican 4 Antibody (51E3) - BSA Free Summary

• Description: Novus Biologicals Mouse Glypican 4 Antibody (51E3) - BSA Free (NBP2-49892) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Glypican 4 (401-529aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPC4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:200
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glypican 4 Antibody (51E3) - BSA Free

• Glypican 4
• glypican proteoglycan 4
• glypican-4
• GPC4
• K-glypican
• K-glypicandJ900E8.1 (glypican 4)

Background

The glypican (Gpc) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the stage of intiation of neural tube closure, suggesting that glypican-4 is unlikely to play an essential role in convergent extension in the mouse

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPC4