Glycogen Synthase Antibody (SN75-05)

Western Blot: Glycogen Synthase Antibody (SN75-05) [NBP2-67693] - Western blot analysis of Glycogen Synthase on A431 lysates using anti- Glycogen Synthase antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Glycogen Synthase Antibody (SN75-05) [NBP2-67693] - Staining Glycogen synthase in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glycogen Synthase Antibody (SN75-05) [NBP2-67693] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glycogen synthase antibody. Counter stained with hematoxylin.

Flow Cytometry: Glycogen Synthase Antibody (SN75-05) [NBP2-67693] - Analysis of 293 cells with Glycogen synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Glycogen Synthase Antibody (SN75-05) [NBP2-67693] - Analysis of Glycogen synthase on Hela lysates using anti- Glycogen synthase antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Glycogen Synthase Antibody (SN75-05) [NBP2-67693] - Staining Glycogen synthase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Glycogen Synthase Antibody (SN75-05) [NBP2-67693] - Staining Glycogen synthase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glycogen Synthase Antibody (SN75-05) [NBP2-67693] - Analysis of paraffin-embedded mouse prostate tissue using anti-Glycogen synthase antibody. Counter stained with hematoxylin.

Product Discontinued

Glycogen Synthase Antibody (SN75-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Glycogen Synthase aa 638-737 / 737. (SwissProt: P13807 Human; SwissProt: Q9Z1E4 Mouse; SwissProt: A2RRU1 Rat)
• Localization: Cytosol, Inclusion body, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GYS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glycogen Synthase Antibody (SN75-05)

• EC 2.4.1.11
• glycogen [starch] synthase, muscle
• glycogen synthase 1 (muscle)
• GYSGSY

Background

Glycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GYS1