Glycogen Phosphorylase BB/GPBB Antibody - BSA Free

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP2-47446] - Analysis in human cell line SK-MEL-30 and human cell line HeLa.

Immunocytochemistry/Immunofluorescence: Glycogen Phosphorylase BB/GPBB Antibody [NBP2-47446] - Staining of human cell line PC-3 shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP2-47446] - Staining of human prostate shows strong positivity in basal layer of glands.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Glycogen Phosphorylase BB/GPBB Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: GTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPP
• Predicted Species: Mouse (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PYGB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Glycogen Phosphorylase BB/GPBB Protein (NBP2-47446PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Glycogen Phosphorylase BB/GPBB Antibody - BSA Free

• Brain glycogen phosphorylase
• EC 2.4.1.1
• Glycogen phosphorylase B
• Glycogen phosphorylase brain form
• Glycogen Phosphorylase Isoenzyme BB
• GPBB
• MGC9213
• Phosphorylase glycogen brain
• PYGB

Background

GPBB is found predominantly in the brain. Glycogen phosphorylase is a key enzyme of glycogenolysis, catalyzing the rate determining step in glycogen degradation. The activity of this enzyme is allosterically activated by AMP and alosterically inhibited by ATP, ADP, and glucose 6 phosphate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PYGB