glycerol-3-phosphate permease Overexpression Lysate

Western Blot: glycerol-3-phosphate permease Overexpression Lysate (Adult Normal) [NBP2-06750] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for glycerol-3-phosphate permease.

• Reactivity: Hu
• Applications: WB

glycerol-3-phosphate permease Overexpression Lysate Summary

• Description: Transient overexpression lysate of solute carrier family 37 (glycerol-3-phosphate transporter), member 1 (SLC37A1) The lysate was created in HEK293T cells, using Plasmid ID RC220514 and based on accession number NM_018964. The protein contains a C-MYC/DDK Tag.
• Gene: SLC37A1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 57.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for glycerol-3-phosphate permease Overexpression Lysate

• FLJ22340
• G-3-P permease
• G-3-P transporter
• G3PPgene similar to glycerol-3-phosphate permease10Glycerol-3-phosphate permease
• glycerol-3-phosphate transporter
• solute carrier family 37 (glycerol-3-phosphate transporter), member 1
• Solute carrier family 37 member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SLC37A1