TMPRSS3 Antibody

Western Blot: TMPRSS3 Antibody [NB100-77342] - (0.1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TMPRSS3 Antibody Summary

• Description: Novus Biologicals Goat TMPRSS3 Antibody (NB100-77342) is a polyclonal antibody validated for use in WB and ELISA. Anti-TMPRSS3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKIVYHSKYKPKR corresponding to internal region according to NP_076927.1, NP_115777.1, NP_115780.1, NP_115781.1.
• Epitope: EKIVYHSKYKPKR
• Specificity: This antibody is expected to recognise all four reported isoforms (NP_076927.1; NP_115777.1; NP_115780.1; NP_115781.1)
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TMPRSS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in A431 lysates (calculated MW of 49.4 kDa band according to NP_076927.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TMPRSS3 Antibody

• DFNB10
• DFNB8
• EC 3.4.21
• EC 3.4.21.-
• ECHOS1
• gene similar to transmembrane serine protease10ECHOS1Serine protease TADG-12
• serine protease TADG12
• TADG12
• TMPRSS3
• transmembrane protease serine 3
• transmembrane protease, serine 3
• Tumor-associated differentially-expressed gene 12 protein

Background

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a serine protease domain, a transmembrane domain, a LDL receptor-like domain, and a scavenger receptor cysteine-rich domain. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified by its association with both congenital and childhood onset autosomal recessive deafness. This gene is expressed in fetal cochlea and many other tissues, and is thought to be involved in the development and maintenance of the inner ear or the contents of the perilymph and endolymph. This gene was also identified as a tumor associated gene that is overexpressed in ovarian tumors. Four alternatively spliced variants have been described, two of which encode identical products.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMPRSS3 Antibody (NB100-77342)(1)

Publication using NB100-77342

• Lee YJ, Park D, Kim SY, Park WJ. Pathogenic mutations but not polymorphisms in congenital and childhood onset autosomal recessive deafness disrupt the proteolytic activity of TMPRSS3. J Med Genet 2003-08-01 [PMID: 12920079]

Bioinformatics

• Gene Symbol: TMPRSS3
• Entrez:
  • 64699(Human)
  • 140765(Mouse)
• Uniprot:
  • NP_076927.1(Human)