Glutathione S-Transferase pi 1/GSTP1 Antibody

Images

 
Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-33627] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-33627] - Paraformaldehyde-fixed HeLa, using GSTP1 (NBP1-33627) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-33627] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Glutathione S-Transferase pi 1/GSTP1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GSTP1. The exact sequence is proprietary.
Predicted Species
Rat (100%), Canine (100%), Monkey (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSTP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glutathione S-Transferase pi 1/GSTP1 Antibody

  • deafness, X-linked 7
  • DFN7
  • EC 2.5.1.18
  • FAEES3
  • fatty acid ethyl ester synthase III
  • glutathione S-transferase P
  • Glutathione STransferase pi 1
  • Glutathione S-Transferase pi 1
  • GST class-pi
  • GST3DFN7
  • GSTP
  • GSTP1
  • GSTP1-1
  • PI

Background

GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, which is located on chromosome 11q13, is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GSTP1
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