Glutathione S-transferase Mu 5 Antibody - Azide and BSA Free

Western Blot: Glutathione S-transferase Mu 5 Antibody [NBP2-97064] - Anti-Glutathione S-transferase Mu 5 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).

Immunohistochemistry-Paraffin: Glutathione S-transferase Mu 5 Antibody [NBP2-97064] - Immunochemical staining of human GSTM5 in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: Glutathione S-transferase Mu 5 Antibody [NBP2-97064] - Immunochemical staining of human Glutathione S-transferase Mu 5 in human kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: Glutathione S-transferase Mu 5 Antibody [NBP2-97064] - Glutathione S-transferase Mu 5 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 0.5 ul anti-Glutathione S-transferase Mu 5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Glutathione S-transferase Mu 5 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 20 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glutathione S-transferase Mu 5 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human Glutathione S-transferase Mu 5 (Accession#: AAH58881.1; Met1-Lys218)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSTM5
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 1-2 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Glutathione S-transferase Mu 5 Antibody - Azide and BSA Free

• EC 2.5.1.18
• glutathione S-alkyltransferase M5
• glutathione S-aralkyltransferase M5
• glutathione S-aryltransferase M5
• glutathione S-transferase M5
• glutathione S-transferase mu 5
• GST class-mu 5
• GSTM5-5
• GTM5
• S-(hydroxyalkyl)glutathione lyase M5

Background

The GSTM5 (glutathione S-transferase Mu 5) gene codes for a 218 amino acid long, 25 kDA protein that functions as a catalyst in various reactions in eukaryotes and prokaryotes such as converting reduced gluthathione to a large number of exogenous and endogenous hydrophobic electrophiles. GSTM5 has been linked to various diseases such as hypertension, hepatitis, adenocarcinoma, breast cancer, coronary heart disease, and hypertension. GSTM5 participates in gluthione metabolism and conjugation as well as metapathway biotransformation and has been known to interact with various genes such as CYP1A1, CYP1B1, CYP2B6, CYP2C19, and ALDH1A3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSTM5