Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c

Western Blot: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - Analysis of Glutathione Peroxidase 4 (Isoform a and c) in Human Testis lysate using this antibody at 0.1ug/ml.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - IHC analysis of Glutathione Peroxidase 4 (Isoform a and c) in paraffin embedded Human Testis using this antibody at 0.3 ug/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c Summary

• Immunogen: Peptide with sequence C-EEPLVIEKDLPHY corresponding to C-Terminus according to NP_002076.2, NP_001034937.1.
• Specificity: This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPX4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.3 ug/ml
  • Immunohistochemistry-Paraffin 0.3 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 19 kDa band observed in human kidney and testis lysates and in HEK293 lysates (calculated MW of 22.1 kDa band according to NP_002076.2). IHC-P: Human testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 4/GPX4 Antibody - Isoform a and c

• EC 1.11.1
• EC 1.11.1.12
• glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Glutathione Peroxidase 4
• GPX4
• GPx-4
• GSHPx-4
• MCSP
• PHGPx
• PHGPxsnGPx
• phospholipid hydroperoxidase
• phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• snGPx
• snPHGPx
• sperm nucleus glutathione peroxidase

Background

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 4/GPX4 Antibody (NB100-41379)(1)

Publication using NB100-41379

• Su D, Novoselov SV, Sun QA et al. Mammalian selenoprotein thioredoxin/glutathione reductase: Roles in disulfide bond formation and sperm maturation. J Biol Chem 2005-05-18 [PMID: 15901730]

Bioinformatics

• Gene Symbol: GPX4
• Entrez:
  • 2879(Human)
• Uniprot:
  • NP_002076.2(Human)