Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free [NBP3-08253] - Confocal immunofluorescence image of HepG2 cells stained with Glutathione Peroxidase 4/GPX4 Mouse Monoclonal Antibody (LHM 2) followed by goat anti- Mouse CF488 (green). RedDot is used to label the nuclei red.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA, Flow, ICC/IF, IHC
• Clone: LHM 2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07344).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: A 375P cells crude extract
• Localization: Mitochondrion, Cytoplasm
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPX4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: ELISA: For coating, order antibody without BSA
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free

• EC 1.11.1
• EC 1.11.1.12
• glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Glutathione Peroxidase 4
• GPX4
• GPx-4
• GSHPx-4
• MCSP
• PHGPx
• PHGPxsnGPx
• phospholipid hydroperoxidase
• phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• snGPx
• snPHGPx
• sperm nucleus glutathione peroxidase

Background

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 4/GPX4 Antibody (NBP3-08253)(1)

Publication using NBP3-08253

• Wang J, Li J, Yin L et Al. MSCs promote the efferocytosis of large peritoneal macrophages to eliminate ferroptotic monocytes/macrophages in the injured endometria Stem Cell Res Ther 2024-05-01 [PMID: 38693589]

Bioinformatics

• Gene Symbol: GPX4