Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

Glutamate Dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24494] - Standard Reference Curve

Glutamate Dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24494] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Serum, plasma, urine and animal tissue
• Standard Curve Range: 0.54 - 25.0 U/L
• Sensitivity: 0.54 U /L

Glutamate Dehydrogenase Activity Assay Kit (Colorimetric) Summary

• Description: Detection instrument: Microplate reader (440 - 460 nm, optimum wavelength: 450 nm)
  Assay time: 35 min
• Standard Curve Range: 0.54 - 25.0 U/L
• Sensitivity: 0.54 U /L
• Inter-Assay: %CV < 4
• Intra-Assay: %CV < 3
• Spike Recovery: Average recovery rate: 103%
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: GLUD1

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

• EC 1.4.1
• EC 1.4.1.3
• GDH 1
• GDH
• GDH1
• GLUD
• glutamate dehydrogenase (NAD(P)+)
• Glutamate Dehydrogenase 1
• glutamate dehydrogenase 1, mitochondrial
• MGC132003

Background

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GLUD1