Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

ELISA: Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence) [NBP2-67990] - Samples were spiked with high concentrations of Human Glutamate Dehydrogenase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence) [NBP2-67990] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.09 mIU/mL

Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human GDH/GLDH in samples. No significant cross-reactivity or interference between Human GDH/GLDH and analogues was observed.
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.09 mIU/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9.65%
• Intra-Assay: CV% < 10.19%
• Spike Recovery: 88-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: GLUD1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

• EC 1.4.1
• EC 1.4.1.3
• GDH 1
• GDH
• GDH1
• GLUD
• glutamate dehydrogenase (NAD(P)+)
• Glutamate Dehydrogenase 1
• glutamate dehydrogenase 1, mitochondrial
• MGC132003

Background

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GLUD1