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Rat Glut4 ELISA Kit (Colorimetric)

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ELISA: Rat Glut4 ELISA Kit (Colorimetric) [NBP2-82176] - Standard Curve Reference
ELISA: Rat Glut4 ELISA Kit (Colorimetric) [NBP2-82176] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

Order Details

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Rat Glut4 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or interference between Rat GLUT4 and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5%
Intra-Assay
CV% < 6%
Spike Recovery
average = 96% for serum; average = 94% for EDTA plasma; average = 90% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SLC2A4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat Glut4 ELISA Kit (Colorimetric)

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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BSA Free
NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - Glut1 in human brain lysate (55 kDa). Antibody at 1:500. Western blot image submitted by a verified customer review.
Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 &amp; 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 &amp; 4). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY licence.
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Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with&amp;nbsp;100&amp;nbsp;ng/mL Recombinant human IGF-1 (Catalog&amp;nbsp;# 291-G1, dark orange filled histogram) for 15&amp;nbsp;minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887) or control antibody (Catalog&amp;nbsp;# AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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beta Antibody
NBP2-12793
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AF2850
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Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on NIH3T3 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).
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Immunocytochemistry/Immunofluorescence: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Analysis of monoclonal antibody to PRKAB1 on HeLa cell. Antibody concentration 10 ug/ml.
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Immunohistochemistry-Paraffin: RPL6 Antibody [NBP2-20216] - Paraffin-embedded mouse stomach. RPL6 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: RPL6 Antibody [NBP2-20216] - Paraffin-embedded rat fore brain. RPL6 antibody dilution: 1:500.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
MED10 Antibody
NBP3-13045
Western Blot: MED10 Antibody [NBP3-13045] - 293T whole cell and nuclear extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MED10 antibody (NBP3-13045) diluted at 1:500.
Immunocytochemistry/Immunofluorescence: MED10 Antibody [NBP3-13045] - MED10 antibody detects MED10 protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED10 protein stained by MED10 antibody (NBP3-13045) diluted at 1:500. Blue: Hoechst 33342 staining.
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Applications: ICC/IF, WB
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(OTI5E6) - Azide and ...
NBP2-74462
Western Blot: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Analysis of Hela cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Analysis of Jurkat cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications: CyTOF-ready, Flow, ICC/IF, WB
Adiponectin/Acrp30
[HRP]
DRP300
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Bioinformatics

Gene Symbol SLC2A4