Rat Glut4 ELISA Kit (Colorimetric)

ELISA: Rat Glut4 ELISA Kit (Colorimetric) [NBP2-82176] - Samples were spiked with high concentrations of Rat Glut4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Glut4 ELISA Kit (Colorimetric) [NBP2-82176] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL

Rat Glut4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or interference between Rat GLUT4 and analogues was observed.
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5%
• Intra-Assay: CV% < 6%
• Spike Recovery: average = 96% for serum; average = 94% for EDTA plasma; average = 90% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SLC2A4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Glut4 ELISA Kit (Colorimetric)

• Glut4
• GLUT-4
• GLUT4Glucose transporter type 4, insulin-responsive
• insulin-responsive glucose transporter type 4
• SLC2A4
• solute carrier family 2 (facilitated glucose transporter), member 4
• solute carrier family 2, facilitated glucose transporter member 4

Background

The stimulation of glucose uptake by insulin in muscle and adipose tissue requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Glut4 ELISA Kit (NBP2-82176). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SLC2A4