Glut4 Antibody (3G10A3)

Images

 
Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.
Immunocytochemistry/ Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HepG2 cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of paraffin-embedded bladder cancer tissues using Glucose Transporter GLUT4 mouse mAb with DAB staining.
Flow Cytometry: Glut4 Antibody (3G10A3) [NBP2-22214] - Flow cytometric analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green) and negative control (purple).
Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter Glut4 Antibody (3G10A3)mAb against HEK293 (1) and Glucose Transporter GLUT4 (AA: 224-353)-hIgGFc transfected HEK293 ...read more
Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - human Glucose Transporter GLUT4 recombinant protein.
Immunocytochemistry/ Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
ELISA: Glut4 Antibody (3G10A3) [NBP2-22214] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
3G10A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Glut4 Antibody (3G10A3) Summary

Immunogen
Purified recombinant fragment of human Glucose Transporter GLUT4 (AA: 224-353 ) expressed in E. Coli.
Localization
Nucleus, Cytoplasm
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SLC2A4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • CyTOF-ready
Theoretical MW
54.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-22214 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 0.5% protein stabilizer.
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Glut4 Antibody (3G10A3)

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Background

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut4 Antibody (NBP2-22214)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Glut4.

Glucose Transporter 4 (GLUT4, SLC2A4)
GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein...  Read full blog post.

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Bioinformatics

Gene Symbol SLC2A4
Entrez