Glut1 Antibody (1084409) [Unconjugated]

Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glut1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line and absent in human PBMC cells using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface of HepG2 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Glut1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Glut1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 1084409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glut1 Antibody (1084409) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
  Accession # P11166
• Specificity: Detects a synthetic peptide corresponding to residues near amino acid 50 of human GLUT1 protein by Direct ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut1 Antibody (1084409) [Unconjugated]

• Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
• CSE
• DYT17
• DYT18
• DYT9
• EIG12
• Glucose transporter type 1, erythrocyte/brain
• Glut1
• GLUT-1
• GLUT1DS
• HepG2 glucose transporter
• HTLVR
• Human T-Cell Leukemia Virus (I and II) Receptor
• MGC141895
• MGC141896
• PED
• SLC2A1
• solute carrier family 2 (facilitated glucose transporter), member 1
• Solute Carrier Family 2 Member 1
• solute carrier family 2, facilitated glucose transporter member 1

Background

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8⁺ T cells, and erythrocytes (1‑5). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
2. Meuckler, M. et al. 1985, Science 229:941.
3. Jones, K.S. et al. 2006, J. Virol. 8291.
4. Takenouchi, N. et al. 2007, J. Virol. 1506.
5. Kinet, S. et al. 2007, Retrovirology 4:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P11166(Human)