Mouse Glucose 6 phosphate isomerase ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Mouse Glucose 6 phosphate isomerase in samples. No Significant cross-reactivity or interference between Mouse Glucose 6 phosphate isomerase and analogues was observed. |
Standard Curve Range |
0.31 - 20 ng/mL |
Sensitivity |
0.19 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 6% |
Intra-Assay |
CV% < 5% |
Spike Recovery |
average = 100% for serum; average = 102% for EDTA plasma; average = 91% for cell culture media |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
GPI |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab
|
Alternate Names for Mouse Glucose 6 phosphate isomerase ELISA Kit (Colorimetric)
Background
Glucose 6 phosphate isomerase belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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