Human Glucose 6 phosphate isomerase ELISA Kit (Colorimetric)

ELISA: Human Glucose 6 phosphate isomerase ELISA Kit (Colorimetric) [NBP2-82189] - Samples were spiked with high concentrations of Human Glucose 6 phosphate isomerase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Glucose 6 phosphate isomerase ELISA Kit (Colorimetric) [NBP2-82189] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 31.25 - 2000pg/mL
• Sensitivity: 18.75 pg/mL

Human Glucose 6 phosphate isomerase ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Glucose 6 phosphate isomerase in samples. No Significant cross-reactivity or interference between Human Glucose 6 phosphate isomerase and analogues was observed.
• Standard Curve Range: 31.25 - 2000pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6%
• Intra-Assay: CV% < 6%
• Spike Recovery: average = 97% for serum; average = 100% for EDTA plasma; average = 101% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GPI

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Glucose 6 phosphate isomerase ELISA Kit (Colorimetric)

• AMFGNPI
• Autocrine motility factor
• DKFZp686C13233
• EC 5.3.1.9
• glucose phosphate isomerase
• glucose-6-phosphate isomerase
• hexose monophosphate isomerase
• hexosephosphate isomerase
• neuroleukin
• NLKSA36
• oxoisomerase
• PGI
• PHI
• Phosphoglucose isomerase
• phosphohexomutase
• Phosphohexose isomerase
• phosphosaccharomutase
• SA-36
• Sperm antigen 36
• sperm antigen-36

Background

Glucose 6 phosphate isomerase belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Glucose 6 phosphate isomerase ELISA Kit (NBP2-82189). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GPI