GLP1 Antibody (8G9) - BSA Free

Immunocytochemistry/Immunofluorescence: Glucagon Antibody (8G9) - BSA Free [NBP2-23506] - Pancreas sections from diabetic mice stained with GLP1 antibody (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia

Glucagon Antibody (8G9) - BSA Free [NBP2-23506] - BSA Free [NBP2-23506]

Sandwich ELISA Detection: Glucagon Antibody (8G9) - BSA Free [NBP2-23506] - The calibration curve with NBP2-23506 being the capture antibody and NBP2-23557 as the biotinylated detection antibody.

Sandwich ELISA Detection: Glucagon Antibody (8G9) - BSA Free [NBP2-23506] - Calibration curve using NBP2-23506 as the capture antibody and NBP2-23560 as the biotinylated detection antibody.

Product Discontinued

GLP1 Antibody (8G9) - BSA Free Summary

• Immunogen: Synthetic GLP-1(7-36)amide coupled to carrier and adsorbed onto aluminum hydroxide gel
• Epitope: C-terminal epitope of GLP-1(7-36)amide
• Specificity: Clone 8G9 is specific for the amidated C-terminus of the peptide and does not react with GLP-1(7-37)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCG
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Sandwich ELISA Detection
• Application Notes: Clone 8G9 reacts with the amidated C-terminus of GLP-1(7-36)amide, GLP-1(9-36)amide and GLP-1(1-36)amide. Clone 8G9 can be used as capture antibody in sandwich ELISA using 4F3or Clone 10 as detection antibody. Clone 8G9 has been used for the immunoblockade of endogenous GLP-1 in rats .

Reactivity Notes

Mammal. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for GLP1 Antibody (8G9) - BSA Free

• glicentin-related polypeptide
• GLP1
• GLP-1
• glucagon-like peptide 1
• GRPP

Background

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCG