GLP1 Antibody (10) - BSA Free

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GLP1 Antibody (10) - BSA Free [NBP1-05180] - BSA Free [NBP1-05180]
ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - Calibration curve using NBP2-23506 as the capture antibody and NBP2-23557 as the biotinylated detection antibody. Image using the Biotin form of this antibody.
ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other ...read more

Product Details

Summary
Reactivity MaSpecies Glossary
Applications WB, ELISA
Clone
10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

GLP1 Antibody (10) - BSA Free Summary

Immunogen
Synthetic GLP-1(7-17)
Epitope
Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide.
Specificity
Binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. Cross-reacts with Liraglutide.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GCG
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:20000
  • Western Blot 1:100-1:2000
Application Notes
ELISA: NBP1-05180 binds free GLP-1(7-37) and free GLP-1(7-36)amide in solution. NBP1-05180 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well. NBP1-05180 binds free exendin-4 in solution.. When measuring "active" GLP-1 NBP1-05180B works as a detection antibody in combination with NBP1-97413. NBP1-05180B can be used as a detection antibody in combination with NBP2-21747 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

Reactivity Notes

The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for GLP1 Antibody (10) - BSA Free

  • glicentin-related polypeptide
  • GLP1
  • GLP-1
  • glucagon-like peptide 1
  • GRPP

Background

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of of GLP-1 is fully conserved in all mammalian species examined so far.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-05180

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Bioinformatics

Gene Symbol GCG