GLE1 Antibody (1D8) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: GLE1 Antibody (1D8) [H00002733-M03] - Analysis of monoclonal antibody to GLE1 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: GLE1 Antibody (1D8) [H00002733-M03] - Detection limit for recombinant GST tagged GLE1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GLE1 Antibody (1D8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GLE1 (NP_001003722.1, 140 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RMKGTEGLRLWQEEQERKVQALSEMASEQLKRFDEWKELKQHKEFQDLREVMEKSSREALGHQEKLKAEHRHRAKILNLKLREAEQQRVKQAEQERLRKEE
• Specificity: Reacts with GLE1 RNA export mediator homolog (yeast).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in WB and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GLE1 Antibody (1D8) - Azide and BSA Free

• GLE1 (yeast homolog)-like, RNA export mediator
• GLE1 RNA export mediator homolog (yeast)
• GLE1LGLE1 RNA export mediator-like (yeast)
• GLE1-like protein
• GLE1-like, RNA export mediator
• hGLE1GLE1 RNA export mediator (yeast)
• LCCS
• LCCS1
• lethal congenital contracture syndrome 1
• nucleoporin GLE1

Background

This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunofluorescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLE1
• Entrez:
  • 2733(Human)
• Uniprot:
  • NP_001003722(Human)