GIT1 Antibody (JU33-39)

Western Blot: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-GIT1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of A549 cells with GIT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of paraffin-embedded human colon cancer tissue using anti-GIT1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of paraffin-embedded human pancreas tissue using anti-GIT1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of paraffin-embedded mouse fallopian tubes tissue using anti-GIT1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of paraffin-embedded rat brain tissue using anti-GIT1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-GIT1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: GIT1 Antibody (JU33-39) [NBP2-75505] - Analysis of paraffin-embedded human pancreas tissue with Rabbit anti-GIT1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

GIT1 Antibody (JU33-39) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human GIT1 aa 79-297 . (SwissProt: Q9Y2X7 Human; SwissProt: Q68FF6 Mouse; SwissProt: Q9Z272 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GIT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:500-1:1000
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GIT1 Antibody (JU33-39)

• ARF GAP GIT1
• ARF GTPase-activating protein GIT1
• CAT1
• CAT-1
• Cool-associated and tyrosine-phosphorylated protein 1
• G protein-coupled receptor kinase interacting ArfGAP 1
• G protein-coupled receptor kinase interactor 1
• G protein-coupled receptor kinase-interactor 1
• GIT1
• GRK-interacting protein 1

Background

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GIT1