GFM1 Antibody

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Western Blot: GFM1 Antibody [NBP1-98458] - Mouse Heart Lysate 1.0ug/ml, Gel Concentration: 12%

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GFM1 Antibody Summary

Immunogen
The immunogen for this antibody is Gfm1 - C-terminal region. Peptide sequence ELRSCTEGKGEYTMEYCRYQPCSPSTQEELINKYLEATGQLPVKKGKAKN.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GFM1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for GFM1 Antibody

  • EFG
  • EFG1FLJ12662
  • EFGM
  • EF-Gmt
  • EGF1
  • FLJ13632
  • FLJ20773
  • G elongation factor, mitochondrial 1
  • G translation elongation factor, mitochondrial
  • GFMCOXPD1
  • hEFG1
  • mEF-G 1
  • mitochondrial elongation factor G
  • mitochondrial elongation factor G1
  • mitochondrial

Background

Gfm1 is a mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Gfm1 catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Gfm1 does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GFM1
Uniprot