Gephyrin/GPHN Antibody - BSA Free

Western Blot: Gephyrin/GPHN Antibody [NBP2-16641] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Gephyrin/GPHN Antibody [NBP2-16641] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: Gephyrin/GPHN Antibody [NBP2-16641] - A431 whole cell extracts using 5 ug of Gephyrin antibody [N2C1], Internal. Western blot analysis was performed using Gephyrin antibody [N2C1], Internal. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Gephyrin antibody [N2C1], Internal detects Gephyrin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Gephyrin antibody [N2C1], Internal detects Gephyrin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Gephyrin stained by Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Gephyrin antibody [N2C1], Internal detects Gephyrin protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Po, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Gephyrin/GPHN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Gephyrin/GPHN Antibody - BSA Free (NBP2-16641) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Gephyrin/GPHN. The exact sequence is proprietary.
• Localization: Cell junction; synapse (By similarity); synapse; postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cytoplasm; cytoskeleton (By similarity);
• Predicted Species: Porcine (100%), Rhesus Macaque (100%), Chicken (100%), Xenopus (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPHN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:10000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (99%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Gephyrin/GPHN Antibody - BSA Free

• GEPH
• Gephyrin
• GPH
• GPHN
• GPHRYN
• KIAA1385GPHGEPH

Background

This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GPHN
• Uniprot:
  • Q9NQX3(Human)