GEMIN6 Knockout HeLa Cell Lysate

Images

 
There are currently no images for GEMIN6 Lysate (NBP2-65130).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

GEMIN6 Knockout HeLa Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 2
Gene
GEMIN6

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for GEMIN6 Knockout HeLa Cell Lysate

  • FLJ23459
  • gem (nuclear organelle) associated protein 6
  • gem-associated protein 6
  • gemin 6
  • gemin-6
  • SIP2

Background

GEMIN6 is part of a large macromolecular complex, localized to both the cytoplasm and the nucleus, that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), GEMIN4 (MIM 606969), and GEMIN5 (MIM 607005).[supplied by OMIM, Jul 2002]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-77177
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, ICC, IF
NBP1-82991
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-62651
Species: Hu
Applications: WB, IHC, IHC-P
NB100-55266
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-1936
Species: Hu, Mu, Rt, Pm, Xp, Ze
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
H00006607-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-20439
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-76798
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, KO
H00079760-D01P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
NBP2-52454
Species: Hu, Rt
Applications: WB, ELISA, Flow, ICC/IF, CyTOF-ready
NBP2-24740
Species: Hu, Mu, Bv, Ca, Eq, Pm
Applications: WB, IHC, IHC-P
AF2850
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP2-22127
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
H00005564-M02
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
NB100-61049
Species: Hu, Po, Ba, SyHa
Applications: WB, IP
NB110-40591
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP2-20881
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84416
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-33923
Species: Hu
Applications: WB, ICC/IF
NBP2-65130
Species: Hu
Applications: WB

Publications for GEMIN6 Lysate (NBP2-65130) (0)

There are no publications for GEMIN6 Lysate (NBP2-65130).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GEMIN6 Lysate (NBP2-65130) (0)

There are no reviews for GEMIN6 Lysate (NBP2-65130). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GEMIN6 Lysate (NBP2-65130) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBP2-65130

Additional GEMIN6 Products

Bioinformatics Tool for GEMIN6 Lysate (NBP2-65130)

Discover related pathways, diseases and genes to GEMIN6 Lysate (NBP2-65130). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GEMIN6 Lysate (NBP2-65130)

Discover more about diseases related to GEMIN6 Lysate (NBP2-65130).
 

Pathways for GEMIN6 Lysate (NBP2-65130)

View related products by pathway.

PTMs for GEMIN6 Lysate (NBP2-65130)

Learn more about PTMs related to GEMIN6 Lysate (NBP2-65130).
 

Research Areas for GEMIN6 Lysate (NBP2-65130)

Find related products by research area.

Blogs on GEMIN6

There are no specific blogs for GEMIN6, but you can read our latest blog posts.

Customers Who Bought This Also Bought

ZEB2 Antibody
NBP1-82991

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GEMIN6 Knockout HeLa Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol GEMIN6