Gemin 4 Antibody (3E1) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Gemin 4 Antibody (3E1) [H00050628-M01] - Analysis of monoclonal antibody to GEMIN4 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: Gemin 4 Antibody (3E1) [H00050628-M01] - Detection limit for recombinant GST tagged GEMIN4 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Gemin 4 Antibody (3E1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Gemin 4 Antibody (3E1) - Azide and BSA Free (H00050628-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Gemin 4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: GEMIN4 (NP_056536, 959 a.a. ~ 1057 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AAGPWVQGPEQDLTQEALFVYTQVFCHALHIMAMLHPEVCEPLYVLALETLTCYETLSKTNPSVSSLLQRAHEQRFLKSIAEGIGPEERRQTLLQKMSS
• Specificity: GEMIN4 - gem (nuclear organelle) associated protein 4 (3E1)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GEMIN4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Gemin 4 Antibody (3E1) - Azide and BSA Free

• component of gems 4
• DKFZP434B131
• DKFZP434D174
• gem (nuclear organelle) associated protein 4
• Gemin-4
• HC56
• HCAP1
• HCC-associated protein 1
• HHRF-1
• p97DKFZp434D174

Background

The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gemin 4 Antibody (H00050628-M01)(1)

Publication using H00050628-M01

• Meier I, Walker M, Matera A et al. Gemin4 is an essential gene in mice, and its overexpression in human cells causes relocalization of the SMN complex to the nucleoplasm. Biol Open. 2018-02-01 [PMID: 29371219]

Bioinformatics

• Gene Symbol: GEMIN4
• Entrez:
  • 50628(Human)
• Uniprot:
  • NP_056536(Human)