Recombinant Mouse GDF-7/BMP-12 Protein

SDS-Page: Recombinant Mouse GDF-7/BMP-12 Protein [NBP2-61338]

• Reactivity: Mu
• Applications: WB, Bioactivity
• Concentration: LYOPH

Recombinant Mouse GDF-7/BMP-12 Protein Summary

• Description: A homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains corresponding to GDF-7/BMP-12 Source: E. coli

  Uniprot ID: P43029

  Amino Acid Sequence: TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2000 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GDF7
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of GDF-7/BMP-12 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Western Blot
• Theoretical MW: 29.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse GDF-7/BMP-12 Protein

• BMP12
• BMP-12
• GDF7
• GDF-7
• growth differentiation factor 7
• growth/differentiation factor 7

Background

GDF7 encodes a member of the bone morphogenetic protein (BMP) family. BMPs belong to the transforming growth factor-beta superfamily of secreted signalling molecules that regulate diverse processes in growth, repair and embryonic development. In mouse, this gene functions as an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GDF7