Recombinant Mouse GDF-7/BMP-12 Protein

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SDS-Page: Recombinant Mouse GDF-7/BMP-12 Protein [NBP2-61338]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Bioactivity
Concentration
LYOPH

Order Details

Recombinant Mouse GDF-7/BMP-12 Protein Summary

Description
A homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.

Source: Escherichia coli.

Amino Acid Sequence:
TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR

Details of Functionality
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2000 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GDF7
Purity
>95%, by SDS-PAGE
Endotoxin Note
Less than 0.1 EU/ug of rMuGDF-7/BMP-12 as determined by LAL method.

Applications/Dilutions

Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Alternate Names for Recombinant Mouse GDF-7/BMP-12 Protein

  • BMP12
  • BMP-12
  • GDF7
  • GDF-7
  • growth differentiation factor 7
  • growth/differentiation factor 7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GDF7
Entrez