Recombinant Human GDF-5/BMP-14 Protein

• Reactivity: Hu
• Applications: Func, Bioactivity
• Concentration: LYOPH

Recombinant Human GDF-5/BMP-14 Protein Summary

• Description: A disulfide-linked homodimeric protein containing two 120 amino acids.
  
  

  Source: Escherichia coli.

  Amino Acid Sequence:
  APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GDF5
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Less than 0.1 EU/ug of GDF-5/BMP-14 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
• Theoretical MW: 27.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human GDF-5/BMP-14 Protein

• BMP14
• BMP-14
• Cartilage-derived morphogenetic protein 1
• cartilage-derived morphogenetic protein-1
• CDMP-1
• CDMP1LAP4
• GDF5
• GDF-5
• growth differentiation factor 5
• growth/differentiation factor 5
• OS5
• radotermin
• SYNS2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GDF5