GCS1 Antibody - BSA Free

Western Blot: GCS1 Antibody [NBP2-14967] - (30 ug whole cell lysate) H12997. 5% SDS PAGE, antibody diluted at 1:1000.

Western Blot: GCS1 Antibody [NBP2-14967] - analysis of GCS1 in (L1) 100 ug of WT MEF cells and (L2) MEFs treated with GCS1 specific siRNA for 48 hours using anti-GCS1 antibody. * indicates the non-specific band recognized by the antibody. Image from verified customer review.

Immunoprecipitation: GCS1 Antibody [NBP2-14967] - Immunoprecipitation was performed by using 3ug of [NBP2-14967] in 2 mg whole cell lysate from WT MEF cells. The precipitated proteins were enriched with Protein A beads. The eluted proteins were analyzed through 12.5% SDS PAGE. Arrow indicates GCS1 band. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GCS1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GCS1 Antibody - BSA Free (NBP2-14967) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-GCS1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GCS1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MOGS
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID:29465311)
  • Immunoprecipitation Validated from a verified customer review.
  • Knockdown Validated Reported in scientific literature (PMID:29465311)
  • Western Blot 1:500-1:3000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29465311).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GCS1 Antibody - BSA Free

• EC 3.2.1.106
• GCS1
• glucosidase I
• mannosyl-oligosaccharide glucosidase
• MOGS
• processing A-glucosidase I

Background

This gene encodes the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor. This protein is located in the lumen of the endoplasmic reticulum. Defects in this gene are a cause of type IIb congenital disorder of glycosylation (CDGIIb). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GCS1 Antibody (NBP2-14967)(1)

Publication using NBP2-14967

• Liu Y, Leslie PL, Jin A et al. p32 regulates ER stress and lipid homeostasis by down-regulating GCS1 expression FASEB J. 2018-07-20 [PMID: 29465311] (ICC/IF, IP, KD, WB, Mouse, Human)

Reviews for GCS1 Antibody (NBP2-14967) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IP Mouse 08/03/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: MEF cells
• Species: Mouse

reviewed by: Verified Customer WB Mouse 08/03/2015

Summary

• Application: Western Blot
• Sample Tested: MEF cells
• Species: Mouse

Bioinformatics

• Gene Symbol: MOGS
• Uniprot:
  • Q13724(Human)