GCS1 Antibody

Western Blot: GCS1 Antibody [NBP2-14967] - (30 ug whole cell lysate) H12997. 5% SDS PAGE, antibody diluted at 1:1000.

Western Blot: GCS1 Antibody [NBP2-14967] - analysis of GCS1 in (L1) 100 ug of WT MEF cells and (L2) MEFs treated with GCS1 specific siRNA for 48 hours using anti-GCS1 antibody. * indicates the non-specific band recognized by the antibody. Image from verified customer review.

Immunoprecipitation: GCS1 Antibody [NBP2-14967] - Immunoprecipitation was performed by using 3ug of [NBP2-14967] in 2 mg whole cell lysate from WT MEF cells. The precipitated proteins were enriched with Protein A beads. The eluted proteins were analyzed through 12.5% SDS PAGE. Arrow indicates GCS1 band. Image from verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GCS1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Glucosidase I. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MOGS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:29465311). GCS1 antibody validated for IP from a verified customer review.
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29465311).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for GCS1 Antibody

• EC 3.2.1.106
• GCS1
• glucosidase I
• mannosyl-oligosaccharide glucosidase
• MOGS
• processing A-glucosidase I

Background

This gene encodes the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor. This protein is located in the lumen of the endoplasmic reticulum. Defects in this gene are a cause of type IIb congenital disorder of glycosylation (CDGIIb). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GCS1 Antibody (NBP2-14967)(1)

Publication using NBP2-14967

• Liu Y, Leslie PL, Jin A et al. p32 regulates ER stress and lipid homeostasis by down-regulating GCS1 expression FASEB J. 2018-07-20 [PMID: 29465311] (ICC/IF, IP, KD, WB, Mouse, Human)

Reviews for GCS1 Antibody (NBP2-14967) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: cynthia morimoto IP Mouse 08/03/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: MEF cells
• Species: Mouse

reviewed by: cynthia morimoto WB Mouse 08/03/2015

Summary

• Application: Western Blot
• Sample Tested: MEF cells
• Species: Mouse

Bioinformatics

• Gene Symbol: MOGS
• Uniprot:
  • Q13724(Human)