GCS1 Antibody

Images

 
Western Blot: GCS1 Antibody [NBP2-14967] - (30 ug whole cell lysate) H12997. 5% SDS PAGE, antibody diluted at 1:1000.
Western Blot: GCS1 Antibody [NBP2-14967] - analysis of GCS1 in (L1) 100 ug of WT MEF cells and (L2) MEFs treated with GCS1 specific siRNA for 48 hours using anti-GCS1 antibody. * indicates the non-specific band ...read more
Immunoprecipitation: GCS1 Antibody [NBP2-14967] - Immunoprecipitation was performed by using 3ug of [NBP2-14967] in 2 mg whole cell lysate from WT MEF cells. The precipitated proteins were enriched with Protein A ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GCS1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Glucosidase I. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MOGS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunoprecipitation
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-14967 in the following applications:

Reactivity Notes

Mouse (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GCS1 Antibody

  • GCS1
  • glucosidase I
  • mannosyl-oligosaccharide glucosidase
  • processing A-glucosidase I

Background

This gene encodes the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor. This protein is located in the lumen of the endoplasmic reticulum. Defects in this gene are a cause of type IIb congenital disorder of glycosylation (CDGIIb). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GCS1 Antibody (NBP2-14967) (0)

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Reviews for GCS1 Antibody (NBP2-14967) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: IP, WB.

Reviews using NBP2-14967:
Filter by Applications
IP
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation GCS1 NBP2-14967
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reviewed by:
Anonymous
IP Mouse 08/03/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedMEF cells
SpeciesMouse

Blocking

Blocking Details-

Primary Anitbody

Dilution Ratio3 ug of GCS1 antibody in 2mg whole cell lysate from WT MEF cells

Details

Detection Notes10 sec exposure
Immunoprecipitation DetailsProtein A beads
Western Blot GCS1 NBP2-14967
Enlarge
reviewed by:
Anonymous
WB Mouse 08/03/2015
View

Summary

ApplicationWestern Blot
Sample TestedMEF cells
SpeciesMouse

Blocking

Blocking Details-

Primary Anitbody

Dilution Ratio1:1000 dilution

Details

Detection Notes-

Comments

CommentsL1: 100 ug of WT MEF cellsL2: MEFs treated with GCS1 specific siRNA for 48 hoursActin antibody was used as a loading control.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GCS1 Antibody (NBP2-14967) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GCS1 Products

GCS1 NBP2-14967

Bioinformatics Tool for GCS1 Antibody (NBP2-14967)

Discover related pathways, diseases and genes to GCS1 Antibody (NBP2-14967). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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PTMs for GCS1 Antibody (NBP2-14967)

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Calculators

Concentration Calculator

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Anonymous
08/03/2015
Application: IP
Species: Mouse

Anonymous
08/03/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MOGS
Uniprot