GCN1L1 Antibody

Western Blot: GCN1L1 Antibody [NB100-97851] - Detection of mouse and rat GCN1L1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GCN1L1 antibody NB100-97851 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: GCN1L1 Antibody [NB100-97851] - Detection of Human GCN1L1 on HeLa whole cell lysate using NB100-97851.

Western Blot: GCN1L1 Antibody [NB100-97851] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

GCN1L1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2621 and 2671 of human general control of amino-acid synthesis 1-like 1 using the numbering given in entry NP_006827.1 (GeneID 10985).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCN1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Simple Western 1:50
  • Western Blot 1:2000 - 1:10000
• Theoretical MW: 293 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GCN1L1 Antibody

• GCN1 (general control of amino-acid synthesis 1, yeast)-like 1
• GCN1 general control of amino-acid synthesis 1-like 1 (yeast)
• GCN1
• GCN1L
• GCN1-like protein 1
• HsGCN1
• KIAA0219hsGCN1
• peroxisome proliferator activated receptor interacting complex protein
• PRIC295
• translational activator GCN1

Background

GCN1L1 (general control of amino-acid synthesis 1 like 1) is structurally similar to GCN1, a yeast factor that associates with ribosomes and participates in translational elongation. Alternative names for GCN1L1 include translational activator GCN1, GCN1-like protein 1, HsGCN1, and KIAA0219.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCN1L1
• Entrez:
  • 10985(Human)
• Uniprot:
  • Q92616(Human)