GCC1 Antibody - BSA Free

Western Blot: GCC1 Antibody [NBP2-16621] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GCC1 Antibody [NBP2-16621] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP2-16621] - Human hepatoma, using GCC1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GCC1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GCC1. The exact sequence is proprietary.
• Localization: Cytoplasm; Golgi apparatus membrane; Peripheral membrane protein
• Predicted Species: Mouse (93%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCC1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rabbit (87%), Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GCC1 Antibody - BSA Free

• FLJ22035
• GCC1P
• GCC88Golgi coiled-coil protein 1
• golgi coiled-coil 1
• GRIP and coiled-coil domain containing 1
• GRIP and coiled-coil domain-containing protein 1
• MGC20706
• peripheral membrane Golgi protein

Background

The protein encoded by this gene is a peripheral membrane protein. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. It may play a role in the organization of trans-Golgi network subcompartment involved with membrane transport. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GCC1
• Uniprot:
  • Q96CN9(Human)