GBF1 Antibody

Western Blot: GBF1 Antibody [NBP2-16619] - Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GBF1 Antibody [NBP2-16619] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: GBF1 Antibody [NBP2-16619] - Human colon carcinoma analysis. Sample: Paraffin-embedded colon carcinoma dilution: 1:500.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GBF1 Antibody Summary

• Description: Novus Biologicals Rabbit GBF1 Antibody (NBP2-16619) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human GBF1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GBF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 206 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GBF1 Antibody

• ARF1GEF
• BFA-resistant GEF 1
• FLJ21263
• GBF1
• golgi brefeldin A resistant guanine nucleotide exchange factor 1
• golgi-specific brefeldin A resistance factor 1
• Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
• KIAA0248
• KIAA0248FLJ21500
• MGC134877
• MGC134878

Background

This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GBF1
• Uniprot:
  • Q92538(Human)