GBF1 Antibody

Images

 
Western Blot: GBF1 Antibody [NBP2-16619] - Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GBF1 Antibody [NBP2-16619] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: GBF1 Antibody [NBP2-16619] - Human colon carcinoma analysis. Sample: Paraffin-embedded colon carcinoma dilution: 1:500.
Immunohistochemistry-Paraffin: GBF1 Antibody [NBP2-16619] - Immunohistochemical analysis of paraffin-embedded Normal Colon, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GBF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human GBF1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GBF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
206 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GBF1 Antibody

  • ARF1GEF
  • BFA-resistant GEF 1
  • FLJ21263
  • golgi brefeldin A resistant guanine nucleotide exchange factor 1
  • golgi-specific brefeldin A resistance factor 1
  • Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
  • KIAA0248FLJ21500
  • MGC134877
  • MGC134878

Background

This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GBF1 Antibody (NBP2-16619) (0)

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Secondary Antibodies

 

Isotype Controls

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GBF1 NBP2-16619

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Bioinformatics

Gene Symbol GBF1
Uniprot