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GBF1 Antibody

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Immunohistochemistry-Paraffin: GBF1 Antibody [NBP2-14039] - Staining of human heart muscle shows cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: GBF1 Antibody [NBP2-14039] - Staining of human heart muscle shows cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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GBF1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: LTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKS
Predicted Species
Mouse (99%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GBF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GBF1 Protein (NBP2-14039PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GBF1 Antibody

  • ARF1GEF
  • BFA-resistant GEF 1
  • FLJ21263
  • GBF1
  • golgi brefeldin A resistant guanine nucleotide exchange factor 1
  • golgi-specific brefeldin A resistance factor 1
  • Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
  • KIAA0248
  • KIAA0248FLJ21500
  • MGC134877
  • MGC134878

Background

GBF1 is a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ADP ribosylation factor 5(ARF5). GBF1 activity is inhibited by brefeldin, an antibiotic that interferes with protein transport. It has been show to be important to golgi assembly and the coordination of bidirectional transport. Due to these characteristics, GBF1 is also called Golgi-specific brefeldin A-resistance GEF 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ARF1 Antibody (1D9)
NB300-505
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of 3T3 cells compared to an isotype control (blue).
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of Hela cells compared to an isotype control (blue).
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of MCF-7 cells compared to an isotype control (blue).
(13 Publications)
Species: Bv, Ca, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
SOS2 Antibody
[Unconjugated]
AF6260
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
SOS1 Antibody
(SOS-01) - BSA Free
NB110-68800
Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Species: Hu, Mu
Applications: ICC/IF, WB
RASGRF1 Antibody
NBP1-85727
Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP1-85727] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: RASGRF1 Antibody [NBP1-85727] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & the Golgi apparatus. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
(1 Review)
(46 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
ARFGEF1 Antibody
NBP1-88840
Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] - Staining of human cerebral cortex shows cytoplasmic immunoreactivity in neuronal cell bodies.
Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] - Staining of human cerebral cortex shows strong positivity in subsets of neurons.
Western Blot: ARFGEF1 Antibody [NBP1-88840] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
ARFGEF2 Antibody
NBP1-88607
Western Blot: ARFGEF2 Antibody [NBP1-88607] - Analysis in human cell line CACO-2 and human cell line MCF-7.
Immunohistochemistry-Paraffin: ARFGEF2 Antibody [NBP1-88607] - Staining of human small intestine shows distinct cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
PEDFR/PNPLA2/ATGL
Antibody [Unconjugate ...
AF5365
Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PEDF R/PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PEDF R/PNPLA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Simple Western lane view shows lysates of mouse adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for PEDF R/PNPLA2 at approximately 59 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PEDF R/PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5365) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
PEDF R/PNPLA2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Sheep Anti-Human/Mouse/Rat PEDF R/PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5365) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(5 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
KLF6 Antibody
AF3499
KLF6 Antibody
(1 Publication)
Species: Hu
Applications: WB
RAB1B Antibody
NBP2-93131
Immunocytochemistry/Immunofluorescence: RAB1B Antibody [NBP2-93131] - Analysis of C6 cells using RAB1B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: RAB1B Antibody [NBP2-93131] - Analysis of NIH-3T3 cells using RAB1B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: RAB1B Antibody [NBP2-93131] - Analysis of U-2 OS cells using RAB1B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
GEF-H1 Antibody
NBP2-21577
Western Blot: GEF-H1 Antibody [NBP2-21577] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract5% SDS-PAGE GEF-H1 antibody dilution: 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: GEF-H1 Antibody [NBP2-21577] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw 264.7 whole cell lysate/extract G. 30 ug C2Cl2 whole cell lysate/extract 5% SDS-PAGEGEF-H1 antibody dilution: 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: GEF-H1 Antibody [NBP2-21577] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract5% SDS-PAGE GEF-H1 antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
SLC2A4RG Antibody
(4C10)
H00056731-M02
Western Blot: SLC2A4RG Antibody (4C10) [H00056731-M02] - SLC2A4RG monoclonal antibody (M02), clone 4C10 Analysis of SLC2A4RG expression in HeLa.
Immunocytochemistry/Immunofluorescence: SLC2A4RG Antibody (4C10) [H00056731-M02] - Analysis of monoclonal antibody to SLC2A4RG on HeLa cell. Antibody concentration 10 ug/ml.
Species: Hu
Applications: ELISA, ICC/IF, WB
LIGHT/TNFSF14
[Unconjugated]
664-LI/CF
1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.
Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.
(18 Publications)
Species: Hu
Applications: BA
ARHGAP4 Antibody
NBP1-88527
Western Blot: ARHGAP4 Antibody [NBP1-88527] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human spleen shows high expression.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
VDP p115 Antibody
(3F4)
H00008615-M03
Immunocytochemistry/Immunofluorescence: VDP p115 Antibody (3F4) [H00008615-M03] - Analysis of monoclonal antibody to USO1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: VDP p115 Antibody (3F4) [H00008615-M03] - Analysis of monoclonal antibody to USO1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml
Western Blot: VDP p115 Antibody (3F4) [H00008615-M03] - Analysis of USO1 expression in HeLa (Cat # L013V1).
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
GOLPH3 Antibody
NBP2-58286
Immunocytochemistry/Immunofluorescence: GOLPH3 Antibody [NBP2-58286] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.
Western Blot: GOLPH3 Antibody [NBP2-58286] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunohistochemistry-Paraffin: GOLPH3 Antibody [NBP2-58286] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Cytohesin-1
Antibody (6G11)
NBP2-22513
Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human tonsil tissue.
Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human breast carcinoma.
Immunohistochemistry: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human placenta tissue.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
PITX3 Antibody
NBP2-85481
Western Blot: PITX3 Antibody [NBP2-85481] - WB Suggested Anti-PITX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate
Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 1ug/ml
Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Type: 293T. Antibody Dilution: 1.0ug/mlPITX3 is supported by BioGPS gene expression data to be expressed in HEK293T
Species: Hu
Applications: IHC, IHC-P, WB
VAP-A Antibody
NBP1-31237
Western Blot: VAP-A Antibody [NBP1-31237] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: VAP-A Antibody [NBP1-31237] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human lung adenocarcinoma, using VAPA antibody at 1:500 dilution.
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
GBF1 Antibody
NBP2-14039
GBF1 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P

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Bioinformatics

Gene Symbol GBF1