GATA-1 Antibody (JA10-32)

Western Blot: GATA-1 Antibody (JA10-32) [NBP2-66862] - Analysis of GATA1 on K562 cell using anti-GATA1 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: GATA-1 Antibody (JA10-32) [NBP2-66862] - Analysis of paraffin-embedded human liver tissue using anti-GATA1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GATA-1 Antibody (JA10-32) [NBP2-66862] - Analysis of paraffin-embedded human breast cancer tissue using anti-GATA1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GATA-1 Antibody (JA10-32) [NBP2-66862] - Analysis of paraffin-embedded human kidney tissue using anti-GATA1 antibody. Counter stained with hematoxylin.

Product Discontinued

GATA-1 Antibody (JA10-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within human GATA-1 aa 1-250. (SwissProt: P15976 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GATA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GATA-1 Antibody (JA10-32)

• Eryf1
• ERYF1GATA-binding protein 1 (globin transcription factor 1)
• GATA binding protein 1 (globin transcription factor 1)
• GATA1
• GATA-1
• GATA-1erythroid transcription factor
• GATA-binding factor 1
• GF-1
• GF1globin transcription factor 1
• NF-E1 DNA-binding protein
• NFE1erythroid transcription factor 1
• transcription factor GATA1
• XLTT

Background

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA1