GATA-1 Antibody (234732) [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 234732
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 525

GATA-1 Antibody (234732) [Janelia Fluor® 525] Summary

• Immunogen: E. coli-derived recombinant human GATA-1
  Met1-Ser413
  Accession # P15976
• Specificity: Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with rhGATA-2 or rhGATA-6.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: GATA1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for GATA-1 Antibody (234732) [Janelia Fluor® 525]

• Eryf1
• ERYF1GATA-binding protein 1 (globin transcription factor 1)
• GATA binding protein 1 (globin transcription factor 1)
• GATA1
• GATA-1
• GATA-1erythroid transcription factor
• GATA-binding factor 1
• GF-1
• GF1globin transcription factor 1
• NF-E1 DNA-binding protein
• NFE1erythroid transcription factor 1
• transcription factor GATA1
• XLTT

Background

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA1