GAT-1/SLC6A1 Antibody

Product Discontinued

GAT-1/SLC6A1 Antibody Summary

• Description: Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence C-EQPQAGSSTSKE corresponding to C-Terminus according to NP_003033.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLC6A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave bands at approx. 50 kDa band and 37 kDa band in human brain (cerebellum) lysates after 2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 67.1 kDa band according to NP_003033.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GAT-1/SLC6A1 Antibody

• GABA Transporter 1
• GABATHG
• GABATR
• GABATRGAT-1
• GABT1
• GAT1
• GAT-1
• GAT1sodium- and chloride-dependent GABA transporter 1
• SLC6A1
• solute carrier family 6 (neurotransmitter transporter, GABA), member 1
• Solute carrier family 6 member 1

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAT-1/SLC6A1 Antibody (NBP2-26059)(1)

Publication using NBP2-26059

• de Krom M, Staal WG, Ophoff RA et al. A common variant in DRD3 receptor is associated with autism spectrum disorder. Biol Psychiatry 2009-04-01 [PMID: 19058789]

Bioinformatics

• Gene Symbol: SLC6A1
• Entrez:
  • 6529(Human)
  • 79212(Rat)