gamma Sarcoglycan Antibody

Product Discontinued

gamma Sarcoglycan Antibody Summary

• Immunogen: Peptide with sequence C-NQQFQINSNDGKP corresponding to internal region according to NP_000222.1.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SGCG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 45 kDa band in human heart lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4 kDa band according to NP_000222.1. The 45 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for gamma Sarcoglycan Antibody

• 35 kDa dystrophin-associated glycoprotein
• 35DAG
• A4
• DAGA4
• DAGA4,35kD dystrophin-associated glycoprotein
• DMDA
• DMDA1
• gamma sarcoglycan
• gamma-Sarcoglycan
• gamma-SG
• LGMD2C
• LGMDR5
• MAM
• MGC130048
• sarcoglycan, gamma (35kD dystrophin-associated glycoprotein)
• sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
• SCARMD2
• SCG3
• SCG3LGMD2C
• TYPE

Background

gamma Sarcoglycan encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gamma Sarcoglycan Antibody (NBP1-47210)(1)

Publication using NBP1-47210

• Rafii MS, Hagiwara H, Mercado ML et al. Biglycan binds to alpha- and gamma-sarcoglycan and regulates their expression during development. J Cell Physiol 2006-11-01 [PMID: 16883602]

Bioinformatics

• Gene Symbol: SGCG
• Entrez:
  • 6445(Human)
• Uniprot:
  • NP_000222.1(Human)