Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)

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ELISA: Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit [NBP1-91179] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma
Standard Curve Range
12.5 - 200 ng/mL
Sensitivity
0.92 ng/mL

Order Details

Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a 90K/Mac-2BP positive serum. There was no detectable cross reactivity with any of the tested proteins.
Standard Curve Range
12.5 - 200 ng/mL
Sensitivity
0.92 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
11.90%
Intra-Assay
5.50%
Spike Recovery
Spiked samples were prepared by adding four different levels of recombinant 90K/Mac-2BP into serum. Recoveries ranged from 95% to 139% with an overall mean recovery of 116%.
Sample Volume
100 uL (1:100 prediluted)
Kit Type
ELISA Kit (Colorimetric)
Gene
LGALS3BP

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aluminium pouch(es) with a Microplate coated with Monoclonal Antibody (murine) to human 90K/Mac-2 BP
  2. Adhesive Plate Covers
  3. 90K/Mac-2 BP Standard, 200ng/mL; 100ng/mL;50ng/mL;25ng/mL; 12.5ng/mL
  4. HRP-Conjugate anti-90K/Mac-2 BP monoclonal (murine) antibody
  5. Sample diluent 10x (buffered protein matrix)
  6. Reagent Labels
  7. Substrate Solution (tetramethyl-benzidine and hydrogen peroxide, stabilized)
  8. Wash Buffer, Concentrate 10x (phosphate-buffered saline with Tween 20)

Alternate Names for Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)

  • 90K
  • Basement membrane autoantigen p105
  • BTBD17B
  • galectin-3-binding protein
  • Galectin3BP
  • Galectin-3BP
  • L3 antigen
  • Lectin galactoside-binding soluble 3-binding protein
  • lectin, galactoside-binding, soluble, 3 binding protein
  • LGALS3BP
  • M2BP
  • Mac-2 BP
  • Mac-2-binding protein
  • MAC2BP
  • MAC-2BP
  • MAC-2-BP
  • serum protein 90K
  • Tumor-associated antigen 90K

Background

90K/Mac-2 BP is a widely expressed secreted 90 kDa human serum glycoprotein, originally identified in the supernatant of human breast cancer cells. It is identical to Mac-2 BP, the ligand for the secreted lactose-galactose specific S-lectin Mac-2 which is highly expressed by cells of the macrophage-monocyte lineage as well as a variety of other cell types. Although the biological functions and possible roles of 90K/Mac-2 BP are largely unknown, some evidence indicates a role in the immunitary defence mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LGALS3BP
Entrez