Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric) Summary
Description |
This kit is an enzyme-linked immunosorbent assay for quantitative detection. |
Specificity |
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a 90K/Mac-2BP positive serum. There was no detectable cross reactivity with any of the tested proteins. |
Standard Curve Range |
12.5 - 200 ng/mL |
Sensitivity |
0.92 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 11.90% |
Intra-Assay |
CV% < 5.50% |
Spike Recovery |
Spiked samples were prepared by adding four different levels of
recombinant 90K/Mac-2BP into serum. Recoveries
ranged from 95% to 139% with an overall mean recovery of
116%. |
Sample Volume |
100 uL (1:100 prediluted) |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
LGALS3BP |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- 90K/Mac-2 BP Standard, 200ng/mL; 100ng/mL;50ng/mL;25ng/mL; 12.5ng/mL
- Adhesive Plate Covers
- Aluminium pouch(es) with a Microplate coated with Monoclonal Antibody (murine) to human 90K/Mac-2 BP
- HRP-Conjugate anti-90K/Mac-2 BP monoclonal (murine) antibody
- Reagent Labels
- Sample diluent 10x (buffered protein matrix)
- Substrate Solution (tetramethyl-benzidine and hydrogen peroxide, stabilized)
- Wash Buffer, Concentrate 10x (phosphate-buffered saline with Tween 20)
|
Alternate Names for Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)
Background
90K/Mac-2 BP is a widely expressed secreted 90 kDa human serum glycoprotein, originally identified in the supernatant of human breast cancer cells. It is identical to Mac-2 BP, the ligand for the secreted lactose-galactose specific S-lectin Mac-2 which is highly expressed by cells of the macrophage-monocyte lineage as well as a variety of other cell types. Although the biological functions and possible roles of 90K/Mac-2 BP are largely unknown, some evidence indicates a role in the immunitary defence mechanism.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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