Home » Galectin-3BP/MAC-2BP/LGALS3BP » Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kits » Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)

Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)

 Submit a Review

Images

 
(
Enlarge)
ELISA: Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit [NBP1-91179] - Standard curve
ELISA: Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit [NBP1-91179] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma, buffered solution
Standard Curve Range
12.5 - 200 ng/mL
Sensitivity
0.92 ng/mL

Order Details

100% Guarantee on every product

Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a 90K/Mac-2BP positive serum. There was no detectable cross reactivity with any of the tested proteins.
Standard Curve Range
12.5 - 200 ng/mL
Sensitivity
0.92 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 11.90%
Intra-Assay
CV% < 5.50%
Spike Recovery
Spiked samples were prepared by adding four different levels of recombinant 90K/Mac-2BP into serum. Recoveries ranged from 95% to 139% with an overall mean recovery of 116%.
Sample Volume
100 uL (1:100 prediluted)
Kit Type
ELISA Kit (Colorimetric)
Gene
LGALS3BP

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 90K/Mac-2 BP Standard, 200ng/mL; 100ng/mL;50ng/mL;25ng/mL; 12.5ng/mL
  2. Adhesive Plate Covers
  3. Aluminium pouch(es) with a Microplate coated with Monoclonal Antibody (murine) to human 90K/Mac-2 BP
  4. HRP-Conjugate anti-90K/Mac-2 BP monoclonal (murine) antibody
  5. Reagent Labels
  6. Sample diluent 10x (buffered protein matrix)
  7. Substrate Solution (tetramethyl-benzidine and hydrogen peroxide, stabilized)
  8. Wash Buffer, Concentrate 10x (phosphate-buffered saline with Tween 20)

Alternate Names for Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)

  • 90K
  • Basement membrane autoantigen p105
  • BTBD17B
  • galectin-3-binding protein
  • Galectin3BP
  • Galectin-3BP
  • L3 antigen
  • Lectin galactoside-binding soluble 3-binding protein
  • lectin, galactoside-binding, soluble, 3 binding protein
  • LGALS3BP
  • M2BP
  • Mac-2 BP
  • Mac-2-binding protein
  • MAC2BP
  • MAC-2BP
  • MAC-2-BP
  • serum protein 90K
  • Tumor-associated antigen 90K

Background

90K/Mac-2 BP is a widely expressed secreted 90 kDa human serum glycoprotein, originally identified in the supernatant of human breast cancer cells. It is identical to Mac-2 BP, the ligand for the secreted lactose-galactose specific S-lectin Mac-2 which is highly expressed by cells of the macrophage-monocyte lineage as well as a variety of other cell types. Although the biological functions and possible roles of 90K/Mac-2 BP are largely unknown, some evidence indicates a role in the immunitary defence mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Galectin-3 [HRP]
DGAL30
(31 Publications)
Species: Hu
Applications: ELISA
Fibronectin
Antibody - BSA Free
NBP1-91258
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.
Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.
(11 Reviews)
(40 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100&amp;nbsp;pg/mL.
(3 Reviews)
(739 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
D6050
(700 Publications)
Species: Hu
Applications: ELISA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(24 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.
Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).
Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The&amp;nbsp;ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog&amp;nbsp;# 140-B1).
(3 Publications)
Species: Hu
Applications: BA
Galectin-1
Antibody [Unconjugate
AF1152
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog&amp;nbsp;# FAB3832P) and either (A) Normal Goat IgG Control (Catalog&amp;nbsp;# AB-108-C) or (B) Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Galectin‑1 at approximately 17&amp;nbsp;kDa (as indicated) using 1&amp;nbsp;µg/mL of Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by 1:50&amp;nbsp;dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the12-230 kDa separation system.
Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7&amp;nbsp;µg/mL Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) overnight at 4&amp;nbsp;°C. Tissue was stained with the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(4 Reviews)
(13 Publications)
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
G3BP1 Antibody
(2F3)
H00010146-M01
ELISA: G3BP1 Antibody (2F3) [H00010146-M01] - Detection limit for recombinant GST tagged G3BP1 is 0.1 ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 1 ug/ml.
(6 Publications)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
LAMP-1/CD107a
Antibody (5E7) - BSA
NBP2-52721
Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - MCF-7 human breast cancer cell line. Secondary antibody goat anti-mouse IgG Fc Dy680 (NBP1-72880). Image from verified customer review.
Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - ICC usage of NBP2-52721. Image submittied via verified customer review.
Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LAMP (5E7) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(2 Reviews)
(4 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
LAMP-2/CD107b
Antibody (H4B4) - BSA ...
NBP2-22217
ELISA: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Anti-LAMP-2/CD107b was used to assess lysates from human SVG-A cells via ELISA, by loading the indicated mass of total protein per well. Antibodies tested were diluted 1:250, and used in conjunction with an HRP-conjugated secondary antibody. Signal was detected by luminescence. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Immunofluorescence: [Alexa Fluor&amp;#174; 647] [NBP2-22217AF647] - T98G glioblastoma cells grown on #1.5 coverglass stained with a conjugated LAMP-2/CD107b (H4B4)AF647 antibody, counterstained with DAPI. Cathepsin D (green) staining also visible. This image was submitted via customer Review. Image using the Alexa Fluor 647 form of this antibody.
Knockout Validated: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP2 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human LAMP-2/CD107b (H4B4) Monoclonal Antibody (Catalog # NBP2-22217) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LAMP-2/CD107b at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
(2 Reviews)
(26 Publications)
Species: Ch, Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
Cyclophilin A
Antibody
NBP1-30993
Western Blot: Cyclophilin A Antibody [NBP1-30993] - Analysis. Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:20000.
Western Blot: Cyclophilin A Antibody [NBP1-30993] - (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:3000
Knockdown Validated: Cyclophilin A Antibody [NBP1-30993] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
(1 Publication)
Species: Ha, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
(9 Reviews)
(41 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
ALCAM/CD166
Antibody [Unconjugate
AF1172
ALCAM/CD166 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF1172) at 1.7&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
ALCAM/CD166 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF1172) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of mouse brain (cerebellumn) tissue, rat brain (cerebellum) tissue, H4‑II‑E‑C3 rat hepatoma cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2&amp;nbsp;µg/mL of Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). A specific band was detected for ALCAM/CD166 at approximately 90-120&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Reviews)
(14 Publications)
Species: Ca, Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
LBP [Biotin]
DY870-05
(31 Publications)
Species: Hu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma .
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(2 Reviews)
(415 Publications)
Species: Hu
Applications: BA
BBS9 Antibody
NBP1-88927
Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Human
Galectin-3BP/MAC-2BP/ ...
NBP1-91179
Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

Publications for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179) (0)

There are no publications for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179) (0)

There are no reviews for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Galectin-3BP/MAC-2BP/LGALS3BP Products

Bioinformatics Tool for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179)

Discover related pathways, diseases and genes to Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179)

Discover more about diseases related to Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179).
 

Pathways for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179)

View related products by pathway.

PTMs for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179)

Learn more about PTMs related to Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179).
 

Research Areas for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179)

Find related products by research area.

Blogs on Galectin-3BP/MAC-2BP/LGALS3BP

There are no specific blogs for Galectin-3BP/MAC-2BP/LGALS3BP, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  Manual - Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol LGALS3BP