Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)


ELISA: Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit [NBP1-91179] - Standard curve

Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma, buffered solution
Standard Curve Range
12.5 - 200 ng/mL
0.92 ng/mL

Order Details

Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric) Summary

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a 90K/Mac-2BP positive serum. There was no detectable cross reactivity with any of the tested proteins.
Standard Curve Range
12.5 - 200 ng/mL
0.92 ng/mL
Assay Type
Sandwich ELISA
CV% < 11.90%
CV% < 5.50%
Spike Recovery
Spiked samples were prepared by adding four different levels of recombinant 90K/Mac-2BP into serum. Recoveries ranged from 95% to 139% with an overall mean recovery of 116%.
Sample Volume
100 uL (1:100 prediluted)
Kit Type
ELISA Kit (Colorimetric)


Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. 90K/Mac-2 BP Standard, 200ng/mL; 100ng/mL;50ng/mL;25ng/mL; 12.5ng/mL
  2. Adhesive Plate Covers
  3. Aluminium pouch(es) with a Microplate coated with Monoclonal Antibody (murine) to human 90K/Mac-2 BP
  4. HRP-Conjugate anti-90K/Mac-2 BP monoclonal (murine) antibody
  5. Reagent Labels
  6. Sample diluent 10x (buffered protein matrix)
  7. Substrate Solution (tetramethyl-benzidine and hydrogen peroxide, stabilized)
  8. Wash Buffer, Concentrate 10x (phosphate-buffered saline with Tween 20)

Alternate Names for Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)

  • 90K
  • Basement membrane autoantigen p105
  • BTBD17B
  • galectin-3-binding protein
  • Galectin3BP
  • Galectin-3BP
  • L3 antigen
  • Lectin galactoside-binding soluble 3-binding protein
  • lectin, galactoside-binding, soluble, 3 binding protein
  • M2BP
  • Mac-2 BP
  • Mac-2-binding protein
  • MAC2BP
  • MAC-2BP
  • MAC-2-BP
  • serum protein 90K
  • Tumor-associated antigen 90K


90K/Mac-2 BP is a widely expressed secreted 90 kDa human serum glycoprotein, originally identified in the supernatant of human breast cancer cells. It is identical to Mac-2 BP, the ligand for the secreted lactose-galactose specific S-lectin Mac-2 which is highly expressed by cells of the macrophage-monocyte lineage as well as a variety of other cell types. Although the biological functions and possible roles of 90K/Mac-2 BP are largely unknown, some evidence indicates a role in the immunitary defence mechanism.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Gene Symbol LGALS3BP