Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)

ELISA: Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit [NBP1-91179] - Standard curve

Product Discontinued

Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a 90K/Mac-2BP positive serum. There was no detectable cross reactivity with any of the tested proteins.
• Standard Curve Range: 12.5 - 200 ng/mL
• Sensitivity: 0.92 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 11.90%
• Intra-Assay: CV% < 5.50%
• Spike Recovery: Spiked samples were prepared by adding four different levels of recombinant 90K/Mac-2BP into serum. Recoveries ranged from 95% to 139% with an overall mean recovery of 116%.
• Sample Volume: 100 uL (1:100 prediluted)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LGALS3BP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (Colorimetric)

• 90K
• Basement membrane autoantigen p105
• BTBD17B
• galectin-3-binding protein
• Galectin3BP
• Galectin-3BP
• L3 antigen
• Lectin galactoside-binding soluble 3-binding protein
• lectin, galactoside-binding, soluble, 3 binding protein
• LGALS3BP
• M2BP
• Mac-2 BP
• Mac-2-binding protein
• MAC2BP
• MAC-2BP
• MAC-2-BP
• serum protein 90K
• Tumor-associated antigen 90K

Background

90K/Mac-2 BP is a widely expressed secreted 90 kDa human serum glycoprotein, originally identified in the supernatant of human breast cancer cells. It is identical to Mac-2 BP, the ligand for the secreted lactose-galactose specific S-lectin Mac-2 which is highly expressed by cells of the macrophage-monocyte lineage as well as a variety of other cell types. Although the biological functions and possible roles of 90K/Mac-2 BP are largely unknown, some evidence indicates a role in the immunitary defence mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Galectin-3BP/MAC-2BP/LGALS3BP ELISA Kit (NBP1-91179). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LGALS3BP