Recombinant Human Galectin-3 Protein

SDS-Page: Human Galectin-3 Protein [NBP2-34881]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human Galectin-3 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 249 amino acids corresponding to Galectin-3 Source: E. coli

  Uniprot ID: P17931

  Amino Acid Sequence: ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYPGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSATGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI

• Details of Functionality: Galectin-3 protein is fully biologically active when compared to standard. The ED50 as determined by its ability to agglutinate human red blood cells is less than 10 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: LGALS3
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Galectin-3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in 1xPBS, 0.02% Tween-20, 3mM beta-ME, pH 7.4.
• Preservative: 5% Trehalose
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human Galectin-3 Protein

• AGE-R3
• Carbohydrate-binding protein 35
• CBP 35,35 kDa lectin
• CBP35
• GAL3
• Gal-3
• galactin-3
• Galactose-specific lectin 3
• Galactoside-binding protein
• GALBPCBP35
• Galectin3
• Galectin-3
• GALIG
• IgE-binding protein
• L29
• L31
• L-31
• Laminin-binding protein
• Lectin L-29
• lectin, galactoside-binding, soluble, 3
• LGALS2
• LGALS3
• Mac-2 antigen
• Mac-2
• MAC2GAL3

Background

Human Galectin-3 also named AGE-R3, CBP35, GAL3, L29, LGALS3, Mac-2, is belonging to the galectins family and it is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Galectin-3 is approximately 30 kDa and, like all galectins, contains a carbohydrate-recognition-binding domain (CRD) of about 130 amino acids that enable the specific binding of Beta-galactosides. Given Galectin-3's broad biological functionality, it has been demonstrated to be involved in cancer, inflammation and fibrosis, heart disease, and stroke. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Human Galectin-3 shares 79% amino acid sequence identity with rat and mouse Galectin-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: LGALS3
• Uniprot:
  • P17931(Mouse)
  • P17931(Mouse)
  • P17931(Mouse)