Galanin R1/GALR1 Antibody

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079] - Analysis of GALR1 (C Term) in human heart lysate (35ug protein in RIPA buffer) using this antibody at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Galanin R1/GALR1 Antibody Summary

• Description: Novus Biologicals Goat Galanin R1/GALR1 Antibody (NB100-57079) is a polyclonal antibody validated for use in WB and ELISA. Anti-Galanin R1/GALR1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HLSDTKENKSRID corresponding to C-Terminus according to NP_001471.1.
• Epitope: HLSDTKENKSRID
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GALR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human heart lysates (calculated MW of 38.9 kDa band according to NP_001471.1). An additional band of unknown identity was also consistently observed at 150 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Galanin R1/GALR1 Antibody

• GAL1
• Galanin R1
• galanin receptor 1
• galanin receptor type 1
• GalaninR1
• GALNR1
• GALNRGALNR1GAL1-R
• GALR1
• GALR-1

Background

The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galanin R1/GALR1 Antibody (NB100-57079)(1)

Publication using NB100-57079

• Henson BS, Neubig RR, Jang I et al. Galanin receptor 1 has anti-proliferative effects in oral squamous cell carcinoma. J Biol Chem 2005-06-17 [PMID: 15767248]

Bioinformatics

• Gene Symbol: GALR1
• Entrez:
  • 2587(Human)
• Uniprot:
  • NP_001471.1(Human)