GADD153/CHOP Antibody (671708)

GADD153 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with PMA and hydrogen peroxide using Mouse Anti-Human GADD153 Monoclonal Antibody (Catalog # MAB7224) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 671708
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GADD153/CHOP Antibody (671708) Summary

• Immunogen: E. coli-derived recombinant human GADD153
  Met1-Ala169
  Accession # P35638
• Specificity: Detects human GADD153 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDIT3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GADD153/CHOP Antibody (671708)

• C/EBP-homologous protein 10
• C/EBP-homologous protein
• CCAAT/enhancer-binding protein homologous protein
• CEBP zeta
• CHOP
• CHOP10 CEBPZ
• CHOP10
• CHOP-10
• CHOPC/EBP zeta
• DDIT3
• DDIT-3
• DNA damage-inducible transcript 3 protein
• DNA-damage-inducible transcript 3
• GADD153
• Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153, also known as CCAAT-enhancer binding protein zeta (C/EBPz), DDIT, and CHOP, is a ubiquitous 29 kDa basic-leucine zipper transcription factor. GADD153 heterodimerization with other C/EBP factors inhibits their activity and alters their DNA sequence recognition. GADD153 inhibits Wnt signaling by dimerizing with the transcription factors TCF-3 and TCF-4. It is upregulated under conditions of cellular stress including DNA damage, oxidative damage, nutrient depletion, ER stress, and mechanical stress, whereupon it promotes the apoptotic response. The transcriptional activity of GADD153 is enhanced by phosphorylation at Ser 78 and Ser 81. Human GADD153 shares 88% and 90% amino acid sequence identity with mouse and rat GADD153, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GADD153/CHOP Antibody (MAB7224)(1)

Publication using MAB7224

• L Zhang, Z Tian, W Li, X Wang, Z Man, S Sun Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis Biosci. Rep., 2017-07-31;0(0):. 2017-07-31 [PMID: 28760844] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: DDIT3
• Uniprot:
  • P35638()