GADD153/CHOP Antibody (671708) [Alexa Fluor® 700]

• Applications: ICC/IF
• Clone: 671708
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

GADD153/CHOP Antibody (671708) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human GADD153
  Met1-Ala169
  Accession # P35638
• Specificity: Detects human GADD153 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

GADD153, also known as CCAAT-enhancer binding protein zeta (C/EBPz), DDIT, and CHOP, is a ubiquitous 29 kDa basic-leucine zipper transcription factor. GADD153 heterodimerization with other C/EBP factors inhibits their activity and alters their DNA sequence recognition. GADD153 inhibits Wnt signaling by dimerizing with the transcription factors TCF-3 and TCF-4. It is upregulated under conditions of cellular stress including DNA damage, oxidative damage, nutrient depletion, ER stress, and mechanical stress, whereupon it promotes the apoptotic response. The transcriptional activity of GADD153 is enhanced by phosphorylation at Ser 78 and Ser 81. Human GADD153 shares 88% and 90% amino acid sequence identity with mouse and rat GADD153, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.