GABPA Overexpression Lysate (Native)


Western Blot: GABPA Overexpression Lysate (Adult Normal) [NBL1-10911] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GABPA.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

GABPA Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC206300 and based on accession number NM_002040. The protein contains a C-terminal DDK tag.
Homo sapiens GA binding protein transcription factor, alpha subunit 60kDa (GABPA), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
51.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for GABPA

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GABPA Overexpression Lysate (Native)

  • E4TF1-60
  • E4TF1ATranscription factor E4TF1-60
  • GA binding protein transcription factor, alpha subunit 60kDa
  • GA-binding protein alpha chain
  • GA-binding protein transcription factor, alpha subunit (60kD)
  • GABP subunit alpha
  • human nuclear respiratory factor-2 subunit alpha, 10
  • NFT2
  • NRF2
  • NRF2A
  • nuclear respiratory factor 2 alpha subunit
  • Nuclear respiratory factor 2 subunit alpha


This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ICC
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Applications: Func-Inh
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P

Publications for GABPA Lysate (NBL1-10911) (0)

There are no publications for GABPA Lysate (NBL1-10911).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GABPA Lysate (NBL1-10911) (0)

There are no reviews for GABPA Lysate (NBL1-10911). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GABPA Lysate (NBL1-10911) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GABPA Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for GABPA Lysate (NBL1-10911)

Discover related pathways, diseases and genes to GABPA Lysate (NBL1-10911). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GABPA Lysate (NBL1-10911)

Discover more about diseases related to GABPA Lysate (NBL1-10911).

Pathways for GABPA Lysate (NBL1-10911)

View related products by pathway.

PTMs for GABPA Lysate (NBL1-10911)

Learn more about PTMs related to GABPA Lysate (NBL1-10911).

Blogs on GABPA.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol GABPA

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-10911 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

GABPA Recombinant Protein
GABPA Antibody (5C8)
GABPA Antibody (5C8)
GABPA Antibody (5C8)