GABA-AR alpha 2 Antibody

Product Discontinued

GABA-AR alpha 2 Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues specific to the alpha 2 subunit conjugated to KLH
• Specificity: Specific for the ~53k alpha 2-subunit of the GABAA receptor in Western blots of rat brain extracts.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRA2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: NB 300-149 may be used for Western blot analysis where a band at ~53 kDa is seen.

Reactivity Notes

Tested in Rat. Mouse reactivity reported in scientific literature (PMID: 20807777). Predicted cross-reactivity based on sequence identity: Bovine (100%), Canine (100%), Human (100%), Mouse (100%) and Non-Human Primate (100%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for GABA-AR alpha 2 Antibody

• GABA A R alpha 2
• GABA(A) receptor subunit alpha-2
• GABAAR alpha 2
• GABAARa2
• GABRA2
• gamma-aminobutyric acid (GABA) A receptor, alpha 2
• gamma-aminobutyric acid receptor subunit alpha-2

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. The alpha 2 subunit has a benzodiazepine type II pharmacology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 2 Antibody (NB300-149)(1)

Publication using NB300-149

• Werner DF, Swihart A, Rau V et al. Inhaled anesthetic responses of recombinant receptors and knockin mice harboring {alpha}2(S270H/L277A) GABAA receptor subunits that are resistant to isoflurane. J Pharmacol Exp Ther. 2010-08-31 [PMID: 20807777] (WB, Mouse)

Bioinformatics

• Gene Symbol: GABRA2
• Entrez:
  • 2555(Human)
  • 14395(Mouse)
  • 289606(Rat)
• Uniprot:
  • P23576(Rat)