GABA-A R gamma 2 Overexpression Lysate

Product Discontinued

GABA-A R gamma 2 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_000807. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens GABA A Receptor gamma 2, mRNA.
• Gene: GABRG2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 49.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GABA-A R gamma 2 Overexpression Lysate

• CAE2
• ECA2
• GABA A R gamma 2
• GABA(A) receptor subunit gamma-2
• GABAAR gamma 2
• GABAARg2
• GABRG2
• gamma-aminobutyric acid (GABA) A receptor, gamma 2
• gamma-aminobutyric acid receptor subunit gamma-2
• GEFSP3

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for GABA-A R gamma 2 Lysate (NBP2-10701). (Showing 1 - 1 of 1 FAQ).

1. I am looking for a "Primary antibody for GABAa receptor gamma 2" raised from antigen whose aa sequence is between exon 5-10. The application will be for "Immunohistochemistry". I would like to know if you have a product to match it?
   • NB300-190 is direceted against GABA A receptor gamma 2, is suitable for use in IHC and uses a fusion protein from the cytoplasmic loop of the gamma 2 subunit as an antigen, so this should suit your needs.

Bioinformatics

• Gene Symbol: GABRG2