G protein beta 4 Antibody - BSA Free

Western Blot: G protein beta 4 Antibody [NBP3-13295] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with G protein beta 4 antibody (NBP3-13295) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP3-13295] - G protein beta 4 antibody detects G protein beta 4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. G protein beta 4 stained by G protein beta 4 antibody (NBP3-13295) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP3-13295] - G protein beta 4 antibody detects G protein beta 4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. G protein beta 4 stained by G protein beta 4 antibody (NBP3-13295) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

G protein beta antibody detects G protein beta protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: G protein beta protein stained by G protein beta antibody (NBP3-13295) diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu, Rt, Bv, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

G protein beta 4 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse G protein beta 4. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Rat (100%), Xenopus (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNB4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for G protein beta 4 Antibody - BSA Free

• G protein beta-4 subunit
• guanine nucleotide binding protein (G protein), beta polypeptide 4
• guanine nucleotide binding protein beta subunit 4
• guanine nucleotide-binding protein subunit beta-4
• Transducin beta chain 4

Background

FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. TISSUE SPECIFICITY: Strongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNB4