G protein beta 4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: G protein beta 4 Antibody [NBP2-14060] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP2-14060] - Staining of human cerebral cortex shows strong positivity in neuropil.

Western Blot: G protein beta 4 Antibody [NBP2-14060] -Analysis in human cell line SK-MEL-30.

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP2-14060] - Staining of human fallopian tube shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP2-14060] -Staining of human kidney shows strong membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: G protein beta 4 Antibody [NBP2-14060] -staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

G protein beta 4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit G protein beta 4 Antibody - BSA Free (NBP2-14060) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: CRLFDLRADQELLLYSHDNIICGITSVAF
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNB4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  G protein beta 4 Protein (NBP2-14060PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for G protein beta 4 Antibody - BSA Free

• G protein beta-4 subunit
• guanine nucleotide binding protein (G protein), beta polypeptide 4
• guanine nucleotide binding protein beta subunit 4
• guanine nucleotide-binding protein subunit beta-4
• Transducin beta chain 4

Background

FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. TISSUE SPECIFICITY: Strongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for G protein beta 4 Antibody (NBP2-14060) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer MiAr Human 12/07/2020

Summary

• Application: Microarray
• Sample Tested: EDTA Plasma
• Species: Human
• Lot: R38668

Bioinformatics

• Gene Symbol: GNB4